Product Details
| Alternative Name: | Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, Deubiquitinating enzyme 21, NEDD8-specific protease |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 60-74 of human USP21. |
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| UniProt ID: | Q9UK80 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Purified by salt precipitation. |
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| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | USP21 is a ubiquitin-specific protease that catalyzes the hydrolysis of ubH2A thereby acting to relieve ubH2A-specific repression. USP21 is also thought to be involved with hepatocyte regeneration. Furthermore, in addition to its deubiquitinylating activity, USP21 is capable of removing NEDD8 from NEDD8 conjugates but has no effect on SUMO conjugates. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of USP21 in HeLa nuclear fraction (10µg) by Western blotting using PAb to USP21 (human) (Prod. No. BML-PW0585) at 1:1'000 dilution. Note: The bands at ~40kDa are likely to be a truncated forms of the USP21 protein as both these and the full length USP21 band are removed by peptide blockade using the antigenic peptide.
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General Literature References
Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation: T. Nakagawa, et al.; Genes Dev.
22, 37 (2008),
Abstract;
Chromatin modifications and their function: T. Kouzarides; Cell
128, 693 (2007),
Abstract;
Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins: L. Gong, et al.; J. Biol. Chem.
275, 14212 (2000),
Abstract;
