Replaces Prod. #: ALX-260-070
- Fluorogenic substrate of calpains and 20S proteasome
- Used to study the activity of calpains and proteosomal degradation
Fluorogenic substrate for 20S proteasome (Prod. No. BML-PW8720) and other chymotrypsin-like proteases, as well as calpains. Ex.: 380nm Em.: 460nm.
Product Details
| Alternative Name: | Suc-LLVY-AMC, Proteasome substrate (fluorogenic), Calpain substrate (fluorogenic) |
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| Sequence: | N-Succinyl-Leu-Leu-Val-Tyr-AMC (AMC=7-amino-4-methylcoumarin) |
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| Formula: | C40 H53 N5 O10 |
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| MW: | 763.9 |
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| CAS: | 94367-21-2 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥97% (HPLC) |
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| Identity: | Determined by ESI-MS. |
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| Solubility: | Soluble in DMSO (at least 5mM). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cytosolic stress granules relieve the ubiquitin-proteasome system in the nuclear compartment: S. Xu, et al.; EMBO J.
42, e111802 (2023),
Abstract;
Design and discovery of novel dipeptide boronic acid ester proteasome inhibitors, an oral slowly-released prodrug for the treatment of multiple myeloma: X. Wang, et al.; Eur. J. Med. Chem.
250, 115187 (2023),
Abstract;
ACTN2 Mutant Causes Proteopathy in Human iPSC-Derived Cardiomyocytes: A.T.L. Zech, et al.; Cells
11, 2745 (2022),
Abstract;
Comprehensive Analysis of Proteasomal Complexes in Mouse Brain Regions Detects ENO2 as a Potential Partner of the Proteasome in the Striatum: N. Esfahanian, et al.; Cell. Mol. Neurobiol.
42, 2305 (2022),
Abstract;
Cystargolide-based amide and ester Pz analogues as proteasome inhibitors and anti-cancer agents
: C.R. Viera, et al.; R. Soc. Open Sci.
9, 220358 (2022),
Abstract;
Innate Variability in Physiological and Omics Aspects of the Beta Thalassemia Trait-Specific Donor Variation Effects: A.T. Anastasiadi, et al.; Front. Physiol.
13, 907444 (2022),
Abstract;
NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma: X. Huang, et al.; Cell Death Dis.
13, 197 (2022),
Abstract;
Neuropeptide signaling and SKN-1 orchestrate differential responses of the proteostasis network to dissimilar proteotoxic insults: H. Boocholez, et al.; Cell Rep.
38, 110350 (2022),
Abstract;
New Metabolites from the Marine Sponge Scopalina hapalia Collected in Mayotte Lagoon: C.S. Hassane, et al.; Marine Drugs
20, 186 (2022),
Abstract;
Sex differences in training-induced activity of the ubiquitin proteasome system in the dorsal hippocampus and medial prefrontal cortex of male and female mice: S.B. Beamish, et al.; Learn. Mem.
29, 302 (2022),
Abstract;
Short-term exposure to an obesogenic diet causes dynamic dysregulation of proteasome-mediated protein degradation in the hypothalamus of female rats: T. McFadden, et al.; Nutr. Neurosci.
13, 1 (2022),
Abstract;
Supplementation with uric and ascorbic acid protects stored red blood cells through enhancement of non-enzymatic antioxidant activity and metabolic rewiring: V.L. Tzounakas, et al.; Redox Biol.
57, 102477 (2022),
Abstract;
The CRTC-CREB axis functions as a transcriptional sensor to protect against proteotoxic stress in Drosophila: Y. Yin, et al.; Cell Death Dis.
13, 688 (2022),
Abstract;
The protease DDI2 regulates NRF1 activation in response to cadmium toxicity: S.T. Ribeiro, et al.; iScience
25, 105227 (2022),
Abstract;
α-mangostin derivative 4e as a PDE4 inhibitor promote proteasomal degradation of alpha-synuclein in Parkinson's disease models through PKA activation: J. Y. Chen, et al.; Phytomedicine
101, 154125 (2022),
Abstract;
A Transgenic Mouse Model of Eccentric Left Ventricular Hypertrophy With Preserved Ejection Fraction Exhibits Alterations in the Autophagy-Lysosomal Pathway: K. Wenzel, et al.; Front. Physiol.
12, 614878 (2021),
Abstract;
Amniotic Fluid Proteasome and Immunoproteasome in the Setting of Intra-Amniotic Infection, Inflammation, and Preterm Birth: C.A. Ware, et al.; Reprod Sci.
28, 2562 (2021),
Abstract;
Low-Grade Systemic Inflammation Interferes with Anabolic and Catabolic Characteristics of the Aged Human Skeletal Muscle: D. Draganidis, et al.; Oxid. Med. Cell. Longev.
2021, 8376915 (2021),
Abstract;
NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling to antagonize interferon signalling: J. Shu, et al.; Emerg. Microbes Infect.
10, 1609 (2021),
Abstract;
Red Blood Cell Proteasome in Beta-Thalassemia Trait: Topology of Activity and Networking in Blood Bank Conditions: A.T. Anastasiadi, et al.; Membranes
11, 716 (2021),
Abstract;
Targeting AML dependency on VCP-mediated DNA repair through a selective second-generation small molecule inhibitor: B. Roux, et al.; Sci. Transl. Med.
13, eabg1168 (2021),
Abstract;
Biological Evaluation and In Silico Study of Benzoic Acid Derivatives from Bjerkandera adusta Targeting Proteostasis Network Modules: K. Georgousaki, et al.; Molecules
25, 666 (2020),
Application(s): Proteasome activity in human fibroblasts lysates.,
Abstract;
Full Text
Cell cycle-dependent localization of the proteasome to chromatin: Y. Kito, et al.; Sci. Rep.
10, 5801 (2020),
Abstract;
Full Text
Gender-specific changes in energy metabolism and protein degradation as major pathways affected in livers of mice treated with ibuprofen: S. Tiwari, et al.; Sci. Rep.
10, 3386 (2020),
Abstract;
Full Text
Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process: C.S. Hassane, et al.; Microorganisms
20, 1262 (2020),
Application(s): Study of proteasome inhibition by microbial extracts.,
Abstract;
Osirisynes G-I, New Long-Chain Highly Oxygenated Polyacetylenes from the Mayotte Marine Sponge Haliclona sp.: P.E. Campos, et al.; Mar. Drugs
18, 350 (2020),
Abstract;
Reproductive Potential of Yeast Cells Depends on Overall Action of Interconnected Changes in Central Carbon Metabolism, Cellular Biosynthetic Capacity, and Proteostasis: R. Maslanka & R. Zadrag-Tecza; Int. J. Mol. Sci.
21, 7313 (2020),
Abstract;
Full Text
Tetrazolium reduction assays under-report cell death provoked by clinically relevant concentrations of proteasome inhibitors: M.A. Harris, et al.; Mol. Biol. Rep.
47, 4849 (2020),
Abstract;
The Function of Drosophila USP14 in Endoplasmic Reticulum Stress and Retinal Degeneration in a Model for Autosomal Dominant Retinitis Pigmentosa: J. Park, et al.; Biology (Basel)
9, 332 (2020),
Abstract;
Full Text
Chemical characterization and cytotoxic potential of an ellagitannin-enriched fraction from Fragaria vesca leaves: J. Liberal, et al.; Arab. J. Chem. 12, 3652 (2019), Application(s): Fluorogenic Substrate,
Exercise training prevents the perivascular adipose tissue-induced aortic dysfunction with metabolic syndrome: E. DeVallance, et al.; Redox Biol.
26, 101285 (2019),
Abstract;
Full Text
Low proteasomal activity in fast skeletal muscle fibers is not associated with increased age-related oxidative damage: R. Fernando, et al.; Exp. Gerontol.
117, 45 (2019),
Abstract;
Males and Females Differ in the Subcellular and Brain Region Dependent Regulation of Proteasome Activity by CaMKII and Protein Kinase A: R.K. Devulapalli, et al.; Neuroscience
418, 1 (2019),
Abstract;
Mass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells: T. Menneteau, et al.; Mol. Cell. Proteomics
18, 744 (2019),
Abstract;
Proline- and Arginine-Rich Peptides as Flexible Allosteric Modulators of Human Proteasome Activity: M. Gizynska, et al.; J. Med. Chem.
62, 359 (2019),
Abstract;
Full Text
Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers: H.M. Ni, et al.; Am. J. Pathol.
189, 1363 (2019),
Abstract;
Ubiquitin proteasome system (UPS) activation in the cardiachypertrophy of hyperthyroidism: C.A. Lino, et al.; Mol. Cell. Endocrinol.
493, 110451 (2019),
Abstract;
ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle: J.D. Fuqua, et al.; FASEB J.
33, 11735 (2019),
Abstract;
Inactivation of USP14 Perturbs Ubiquitin Homeostasis and Delays the Cell Cycle in Mouse Embryonic Fibroblasts and in Fruit Fly Drosophila: J.H. Lee, et al.; Cell. Physiol. Biochem.
47, 67 (2018),
Abstract;
Full Text
Interactive effects of osmotic stress and burrowing activity on protein metabolism and muscle capacity in the soft shell clam Mya arenaria: F. Haider, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol.
228, 81 (2018),
Abstract;
mTORC1 accelerates retinal development via the immunoproteasome: J.H. Choi, et al.; Nat. Commun.
9, 2502 (2018),
Abstract;
Full Text
A leptospiral AAA+ chaperone-Ntn peptidase complex, HslUV, contributes to the intracellular survival of Leptospira interrogans in hosts and the transmission of leptospirosis: S.L. Dong, et al.; Emerg. Microbes Infect.
6, e105 (2017),
Abstract;
Full Text
A lowered 26S proteasome activity correlates with mantle lymphoma cell lines resistance to genotoxic stress: K. Ben Younes, et al.; BMC Cancer
17, 538 (2017),
Abstract;
Full Text
Affinity purification of human m-calpain through an intrinsically disordered inhibitor, calpastatin: H.H. Nguyen, et al.; PLoS One
12, e0174125 (2017),
Abstract;
Full Text
Label-free Proteomic Analysis of Exosomes Derived from Inducible Hepatitis B Virus-Replicating HepAD38 Cell Line: X. Jia, et al.; Mol. Cell. Proteomics
16, S144 (2017),
Abstract;
Full Text
Neurotoxicity of the steroidal alkaloids tomatine and tomatidine is RIP1 kinase- and caspase-independent and involves the eIF2α branch of the endoplasmic reticulum: D.C. da Silva, et al.; J. Steroid Biochem. Mol. Biol.
171, 178 (2017),
Abstract;
Proteasome Activation by Small Molecules: Y. Leestemaker, et al.; Cell Chem. Biol.
24, 725 (2017),
Abstract;
Proteasome properties of hemocytes differ between the whiteleg shrimp Penaeus vannamei and the brown shrimp Crangon crangon (Crustacea, Decapoda): S. Gotze, et al.; Cell Stress Chaperones
22, 879 (2017),
Abstract;
Full Text
The Architecture of the Anbu Complex Reflects an Evolutionary Intermediate at the Origin of the Proteasome System: A.C.D. Fuchs, et al.; Structure
25, 834 (2017),
Abstract;
Full Text
The effect of ARC ablation on skeletal muscle morphology, function, and apoptotic signaling during aging: K. Vorobej, et al.; Exp. Gerontol.
101, 69 (2017),
Abstract;
Akt/mTOR pathway contributes to skeletal muscle anti-atrophic effect of aerobic exercise training in heart failure mice: V.N. Aline, et al.; Int. J. Cardiol.
214, 137 (2016),
Application(s): Chymotrypsin-like activity of proteasome was assayed,
Abstract;
Cardiac proteasome functional insufficiency plays a pathogenic role in diabetic cardiomyopathy: J. Li, et al.; J. Mol. Cell. Cardiol.
102, 53 (2016),
Abstract;
Chloroquine enhances TRAIL-mediated apoptosis through up-regulation of DR5 by stabilization of mRNA and protein in cancer cells: E.J. Park, et al.; Sci. Rep.
6, 22921 (2016),
Application(s): Measured Chymotryptic proteasome activities,
Abstract;
CNS uptake of bortezomib is enhanced by P-glycoprotein inhibition: Implications for spinal muscular atrophy: E. Foran, et al.; Neurobiol. Dis.
88, 118 (2016),
Application(s): Proteasome assay,
Abstract;
Data on skeletal muscle apoptosis, autophagy, and morphology in mice treated with doxorubicin: T.L. Campbell, et al.; Data Brief
7, 786 (2016),
Application(s): Measured calpain activity in mixed gastrocnemius homogenates,
Abstract;
Influence of the APOE genotype on hepatic stress response: Studies in APOE targeted replacement mice and human liver cells: J. Dose, et al.; Free Radic. Biol. Med.
96, 264 (2016),
Application(s): Hepatic proteasome activity,
Abstract;
Pim1 inhibition as a novel therapeutic strategy for Alzheimer’s disease: R. Velazquez, et al.; Mol. Neurodegener.
11, 52 (2016),
Application(s): Proteasome activity, brain homogenate,
Abstract;
Full Text
Prion-mediated neurodegeneration is associated with early impairment of the ubiquitin-proteasome system: C. McKinnon, et al.; Acta Neuropathol.
131, 411 (2016),
Application(s): Determined chymotrypsin-like activity,
Abstract;
Full Text
6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression: M.A. Han, et al.; Chem. Biol. Interact.
228, 69 (2015),
Application(s): Assay,
Abstract;
A potent and selective inhibitor for the UBLCP1 proteasome phosphatase: Y. He, et al.; Bioorg. Med. Chem.
23, 2798 (2015),
Application(s): Fluorogenic Substrate,
Abstract;
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine
2, 642 (2015),
Application(s): Fluorescence,
Abstract;
Dietary Tocotrienol/γ-Cyclodextrin Complex Increases Mitochondrial Membrane Potential and ATP Concentrations in the Brains of Aged Mice: A. Schloesser, et al.; Ox. Med. Cell. Longev.
2015, Article ID 789710 (2015),
Application(s): Cell Culture, Microplate reader,
Abstract;
Full Text
FV-162 is a novel, orally bioavailable, irreversible proteasome inhibitor with improved pharmacokinetics displaying preclinical efficacy with continuous daily dosing: Z. Wang, et al.; Cell Death Dis.
6, e1815 (2015),
Application(s): Proteasome activity assays,
Abstract;
Full Text
Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts: A.D. Sklirou, et al.; Redox Biol.
5, 205 (2015),
Application(s): Fluorescent detection,
Abstract;
Impact of Leucine Supplementation on Exercise Training Induced Anti-Cardiac Remodeling Effect in Heart Failure Mice: W.M.A. Monteiro de Moraes, et al.; Nutrients
7, 3751 (2015),
Application(s): Assay,
Abstract;
Full Text
NEDD8 Ultimate Buster-1 Long (NUB1L) Protein Suppresses Atypical Neddylation and Promotes Proteasomal Degradation of Misfolded Proteins: J. Li, et al.; J. Biol. Chem.
290, 23850 (2015),
Application(s): Proteasomal peptidase activity assay,
Abstract;
Full Text
Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential: J.K. Patra, et al.; Int. J. Nanomedicine
10, 7253 (2015),
Application(s): Estimate the Chymotrypsin-like proteasomal activity of the ANPs,
Abstract;
Full Text
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype: V.A. Fajardo, et al.; Dis. Model Mech.
8, 999 (2015),
Application(s): Fluorescence,
Abstract;
Full Text
Troxerutin inhibits 2,2',4,4'-tetrabromodiphenyl ether (BDE-47)-induced hepatocyte apoptosis by restoring proteasome function: Z.F. Zhang, et al.; Toxicol. Lett.
233, 246 (2015),
Application(s): Assay,
Abstract;
BAG3 induces the sequestration of proteasomal clients into cytoplasmic puncta: implications for a proteasome-to-autophagy switch: M. Minoia, et al.; Autophagy
10, 1603 (2014),
Abstract;
Full Text
scyllo-Inositol promotes robust mutant Huntingtin protein degradation: A.Y. Lai, et al.; J. Biol. Chem.
289, 3666 (2014),
Abstract;
Full Text
Tau deletion impairs intracellular β-amyloid-42 clearance and leads to more extracellular plaque deposition in gene transfer models: I. Lonskaya, et al.; Mol. Neurodegener.
9, 46 (2014),
Abstract;
Full Text
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget
5, 6252 (2014),
Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay,
Abstract;
Full Text
Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis.
4, e761 (2013),
Application(s): Fluorogenic substrate for kinetics assay of 20S proteasome,
Abstract;
Full Text
STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy: J.V. Ferreira, et al.; Autophagy
9, 1349 (2013),
Abstract;
Protein carbonylation and aggregation precede neuronal apoptosis induced by partial glutathione depletion: A. Dasgupta, et al.; ASN Neuro
4, e00084 (2012),
Abstract;
Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
300, R531 (2011),
Abstract;
Full Text
General Literature References
Reovirus-induced apoptosis is preceded by increased cellular calpain activity and is blocked by calpain inhibitors.: R.L. Debiasi et al.; J. Virol.
73, 695 (1999),
Abstract;
Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes.: L.C. Dang et al.; Biochemistry
37, 1868 (1998),
Abstract;
An alpha-mercaptoacrylic acid derivative is a selective nonpeptide cell-permeable calpain inhibitor and is neuroprotective.: K.K. Wang et al.; Proc. Natl. Acad. Sci. USA
93, 6687 (1996),
Abstract;
Kinetic characterization of the chymotryptic activity of the 20S proteasome.: R.L. Stein et al.; Biochemistry
35, 3899 (1996),
Abstract;
Modulation of hypoxia-induced calpain activity in rat renal proximal tubules.: C.L. Edelstein et al.; Kidney Int.
50, 1150 (1996),
Abstract;
Kinetic studies of isopeptidase T: modulation of peptidase activity by ubiquitin.: R.L. Stein et al.; Biochemistry
34, 12616 (1995),
Abstract;
Purification and characterization of a Z-Leu-Leu-Leu-MCA degrading protease expected to regulate neurite formation: a novel catalytic activity in proteasome.: S. Tsubuki et al.; Biochem. Biophys. Res. Commun.
196, 1195 (1993),
Abstract;
Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates.: T. Sasaki et al.; J. Biol. Chem.
259, 12489 (1984),
Abstract;
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