Replaces Prod. #: ALX-260-039
Irreversible and cell permeable broad-spectrum caspase inhibitor. Inhibits Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). For cell free assays use Prod. No. ALX-260-020.
Product Details
| Alternative Name: | Caspase family inhibitor (fluoromethylketone) |
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| Sequence: | Z-Val-Ala-Asp(OMe)-fluoromethylketone |
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| Formula: | C22H30N3O7F |
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| MW: | 467.5 |
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| CAS: | 187389-52-2 |
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| Purity: | ≥95% |
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| Appearance: | White to off-white solid. |
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| Application Notes: | Irreversible, cell permeable, broad spectrum caspase inhibitor. Limited Fas-induced mouse liver damage and apoptosis to <1%, at a dose of 50µmol/kg. At 50µM, abrogated 7-hydroxystaurosporine and etoposide-induced apoptosis in HL60 cells. |
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| Solubility: | Soluble in DMSO (>10mg/ml), dimethyl formamide (7mg/ml) or 100% ethanol (1.3mg/ml). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for up to 1 year after receipt when stored at -20°C. Stock solutions should be divided into aliquots and stored at -20°C for up to 1 month. |
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| Handling: | Keep cool and dry. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The colonic pathogen Entamoeba histolytica activates caspase-4/1 that cleaves the pore-forming protein gasdermin D to regulate IL-1β secretion: S. Wang, et al.; PLoS Pathog.
18, e1010415 (2022),
Abstract;
The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases: M. D. C. Conde-Rubio, et al.; Cell Death Discov.
7, 164 (2021),
Abstract;
The Wnt Target Protein Peter Pan Defines a Novel p53-independent Nucleolar Stress-Response Pathway: A.S. Pfister, et al.; J. Biol. Chem.
290, 10905 (2017),
Abstract;
Full Text
Digitoxin enhances the growth inhibitory effects of thapsigargin and simvastatin on ER negative human breast cancer cells : L.S. Einbond, et al.; Fitoterapia
109, 146 (2015),
Application(s): Cell culture,
Abstract;
Nitric oxide-induced mitochondrial fission is regulated by dynamin-related GTPases in neurons: M.J. Barsoum; EMBO J.
25, 3900 (2006),
Abstract;
Apoptosis in Alzheimer’s disease--an update: S. Shimohama; Apoptosis
5, 9 (2000),
Abstract;
Protective effect of a caspase inhibitor in models for cerebral ischemia in vitro and in vivo: C. Wiessner, et al.; Cell Mol. Biol. (Noisy-Le-Grand)
46, 53 (2000),
Abstract;
Serine protease inhibitors suppress cytochrome c-mediated caspase-9 activation and apoptosis during hypoxia-reoxygenation: Z. Dong, et al.; Biochem. J.
347, 669 (2000),
Abstract;
Full Text
A specific inhibitor of apoptosis decreases tissue injury after intestinal ischemia-reperfusion in mice: A. Farber, et al.; J. Vasc. Surg.
30, 752 (1999),
Abstract;
Full Text
Correlation of tumor necrosis factor α (TNF α) with high Caspase 3-like activity in myelodysplastic syndromes: S.D. Mundle, et al.; Cancer Lett.
140, 201 (1999),
Abstract;
Monocytic cell necrosis is mediated by potassium depletion and caspase-like protease: M. Warny & C.P. Kelly; Am. J. Physiol.
276, C717 (1999),
Abstract;
Full Text
TNF-induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk: P.F. Piguet, et al.; Lab. Invest.
79, 495 (1999),
Abstract;
Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver: J.M. Chandler, et al.; J. Biol. Chem.
273, 10815 (1998),
Abstract;
Full Text
Activation of PKCalpha downstream from caspases during apoptosis induced by 7-hydroxystaurosporine or the topoisomerase inhibitors, camptothecin and etoposide, in human myeloid leukemia HL60 cells: R.G. Shao, et al.; J. Biol. Chem.
272, 31321 (1997),
Abstract;
Full Text
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J.
315, 21 (1996),
Abstract;
Full Text
Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli: A. Sarin, et al.; J. Exp. Med.
184, 2445 (1996),
Abstract;
An ICE-like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells: H. Zhu, et al.; FEBS Lett.
374, 303 (1995),
Abstract;
Involvement of multiple proteases during Fas-mediated apoptosis in T lymphocytes: S.C. Chow, et al.; FEBS Lett.
364, 134 (1995),
Abstract;
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