Fluorogenic substrate for ADAM8 (kcat/Km = 1.0 x 105 M-1s-1), ADAM10 (6.2 x 103), ADAM12 (2.8 x 105), TACE (TNF-α converting enzyme, ADAM17) (4.3 x 105), and likely other ADAMs, especially those that cleave TNF-α type sequences. Also efficiently cleaved by MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-13, and MMP-14. FAM fluorescence is quenched by the Dabcyl group until cleavage separates them. Ex.: 485nm, Em.: 530nm. This substrate is useful for inhibitor screening and kinetic analysis.
Product Details
| Sequence: | Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(5-FAM)-NH2 . TFA (Dabcyl=4-(4-dimethylaminophenylazo)benzoyl; FAM=carboxyfluorescein) |
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| MW: | 1542.5 |
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| Quantity: | 1mg net peptide. |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Pink powder. |
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| Solubility: | Soluble in DMSO (≥10mM) or TRIS, pH 8.0 assay buffer (≥10μM). |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solutions in DMSO are stable for 3 months at -20°C. |
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| Handling: | Protect from light and moisture. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A substrate-optimized electrophoretic mobility shift assay for ADAM12: A. Kotzsch, et al.; Anal. Biochem.
452, 34 (2014),
Abstract;
Catalytic properties of ADAM12 and its domain deletion mutants: J. Jacobsen, et al.; Biochemistry
47, 537 (2008),
Abstract;
Heparan sulfate regulates ADAM12 through a molecular switch mechanism: H.P. Sorensen, et al.; J. Biol. Chem.
283, 31920 (2008),
Abstract;
Full Text
Fluorescent substrates for the proteinases ADAM17, ADAM10, ADAM8, and ADAM12 useful for high-throughput inhibitor screening: M.L. Moss & F.H. Rasmussen; Anal. Biochem.
366, 144 (2007),
Abstract;
