Sensitive fluorogenic substrate for cathepsin K (Km=8µM, kcat/Km=4 x 10 M-1s-1). This substrate is also cleaved by cathepsin B, F, L, L2/V, S. Z-LR-AMC is also cleaved by malaria parasite cysteine proteases falcipain-1, -2, -3, berghepain, and vivapain-2, -3. Ex.: 365nm, Em.: 440nm, although the following Ex/Em can also be used: 355,380/450,460. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144).
Product Details
Alternative Name: | Z-Leu-Arg-AMC, Cathepsin K substrate (fluorogenic) |
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Sequence: | Z-Leu-Arg-AMC . HCl [Z=Cbz=Benzyloxycarbonyl; AMC=7-amino-4-methylcoumarin] |
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MW: | 578.7 |
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Source: | Synthetic. |
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Formulation: | Lyophilized. |
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Purity: | ≥97% (HPLC) |
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Appearance: | White to off-white solid. |
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Solubility: | Soluble in DMSO to at least 20mM. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Use/Stability: | Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. |
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Handling: | Protect from light and moisture. |
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Regulatory Status: | RUO - Research Use Only |
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