Fluorogenic substrate specific for cathepsin B (kcat/Km=105 M-1s-1). The following proteases do not cleave, or inefficiently cleave, this substrate: papain, cathepsins C/DPP-I, F, H, K/O2, L, L2/V, O, S, X/Z. Ex.: 365 nm, Em.: 440 nm, although the following Ex/Em can also be used: 355,380/450,460. This substrate is useful for analysis of cathepsin B activity in cell extracts, inhibitor screening, and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144).
Product Details
Alternative Name: | Z-Arg-Arg-AMC |
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Sequence: | Z-Arg-Arg-AMC . HCl [Z=Cbz=Benzyloxycarbonyl; AMC=7-amino-4-methylcoumarin] |
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MW: | 621.7 |
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CAS: | 88937-61-5 |
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Purity: | ≥98% |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Abstract;
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Abstract;
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Abstract;
BCM-95 and (2-hydroxypropyl)-β-cyclodextrin reverse autophagy dysfunction and deplete stored lipids in Sap C-deficient fibroblasts: M. Tatti, et al.; Hum. Mol. Genet.
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Abstract;
Ascorbic acid modulation of iron homeostasis and lysosomal function in trabecular meshwork cells: P. Xu, et al.; J. Ocul. Pharmacol. Ther.
30, 246 (2014),
Abstract;
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Cannabinoid receptor 1 modulates the autophagic flux independent of mTOR- and BECLIN1-complex: C. Hiebel, et al.; J. Neurochem.
131, 484 (2014),
Abstract;
Cathepsin B is up-regulated and mediates extracellular matrix degradation in trabecular meshwork cells following phagocytic challenge: K. Porter, et al.; PLoS One
8, e68668 (2013),
Abstract;
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