Fluorogenic substrate for several matrix metalloproteinases (MMPs). It is cleaved by MMP-1, MMP-3, MMP-7, MMP-8, MMP-9, MMP-11, MMP-12, MMP-13, and ADAM9/MDC9, but only slightly by MMP-2 and not at all by ADAM17/TACE. Ex/Em= 340nm/440nm, although 365/450 is also appropriate. Highly quenched, with strong fluorescence by Nma at 440nm once cleavage separates it from the aromatic Dnp moiety. Compatible with most fluorometers.
Product Details
Alternative Name: | Matrix metalloproteinase substrate, Dnp-Pro-β-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(Nma)-NH2 |
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Sequence: | Dnp-P(Cha)G(CMe)HA(KNma)-amide |
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MW: | 1077.4 |
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Purity: | ≥98% (HPLC) |
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Appearance: | lyophylized solid |
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Solubility: | Soluble in DMSO (at least 20mM). |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Handling: | Protect from light. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Application(s): Substrate in MMP-1 activity assays.,
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Toll-Like Receptor 4-Independent Carbon Tetrachloride-Induced Fibrosis and Lipopolysaccharide-Induced Acute Liver Injury in Mice: Role of Hepatic Stellate Cells: S. Kumar, et al.; Am. J. Pathol.
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The tumor necrosis factor-alpha converting enzyme (TACE): a unique metalloproteinase with highly defined substrate selectivity: M.J. Mohan, et al.; Biochemistry
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