The FLUOR DE LYS®-Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the FLUOR DE LYS®-Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm). The FLUOR DE LYS®-Succinyl Green, Desuccinylase Substrate has longer wavelength excitation and emission than the AMC-based FLUOR DE LYS®-Succinyl, Desuccinylase Substrate (Prod. No. BML-KI590). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.
Product Details
| Source: | Synthetic. |
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| Concentration: | 5 mM |
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| Formulation: | Solution in DMSO. |
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| Excitation maximum: | 470-500nm |
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| Emission maximum: | 520-550nm |
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| Purity: | ≥95% (TLC) |
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| Appearance: | Liquid. |
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| Application Notes: | Can be used for the study of desuccinylase kinetics, regulation, and inhibitor sensitivity. Ideal for drug discovery and HTS applications. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for 1 year when stored at -80°C. |
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| Technical Info/Product Notes: | NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105). For assay conditions, see the product manual for BML-AK514 Green SIRT5 Fluorimetric Drug Discovery Kit. |
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| Regulatory Status: | RUO - Research Use Only |
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