The FLUOR DE LYS®-Green substrate offers a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS®-Green Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a fluorophore. The FLUOR DE LYS®-Green substrate has a higher wavelength excitation and emission than the original FLUOR DE LYS® substrate (Prod. No. BML-KI104). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.
Product Details
| Quantity: | 2.5 µmol |
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| Formulation: | 50mM solution in DMSO. |
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| Purity: | ≥95% (HPLC) |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Technical Info/Product Notes: | NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105; see attached assay conditions). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Biphenyl-4-yl-acrylohydroxamic acids: Identification of a novel indolyl-substituted HDAC inhibitor with antitumor activity: R. Cincinelli, et al.; Eur. J. Med. Chem.
112, 99 (2016),
Abstract;
4-(1-Ethyl-4-anisyl-imidazol-5-yl)-N-hydroxycinnamide–A new pleiotropic HDAC inhibitor targeting Cancer cell signalling and cytoskeletal organisation: K. Mahal, et al.; Exp. Cell Res.
336, 263 (2015),
Application(s): HDAC Assay,
Abstract;
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