FLUOR DE LYS®-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. FLUOR DE LYS®-SIRT2 deacetylase substrate is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate (Prod. No. BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with FLUOR DE LYS® Developer II (Prod. No. BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (Prod. No. BML-SE251; 2U/well, 100 µM substrate).
Product Details
| Formulation: | Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer. |
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| Purity: | ≥95% (HPLC) |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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2, 100313 (2021),
Application(s): HTS protocol to identify deacylase activities,
Abstract;
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The Contribution of Romidepsin to the Herbicidal Activity of Burkholderia rinojensis Biopesticide: D.K. Owens, et al.; J. Nat. Prod.
83, 843 (2020),
Abstract;
Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem.
62, 5844 (2019),
Abstract;
Pharmacophore modeling and virtual screening studies to identify novel selective SIRT2 inhibitors: G. Eren, et al.; J. Mol. Graph. Model.
10, 1313 (2019),
Abstract;
