- High potency retained following inactivation
- Minimal lot-to-lot variation
- Lyophilized for ease of handling and long term stability
Extremely potent cytotoxin.
Product Details
| Alternative Name: | Clostridium difficile toxin B |
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| Source: | From Clostridium difficile, strain VPI10463. |
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| UniProt ID: | P18177 |
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| Formulation: | Lyophilized. Contains 0.05M HEPES, 0.15M sodium chloride and 5% sucrose. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified protein. |
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| Appearance: | Clear residue. |
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| Biological Activity: | 2 pg/ml by verocytotoxicity assay (lowest point at which cell rounding is visible) |
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| Applications: | ELISA
Antibody capture, Cytotoxicity studies, LINE assay
|
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| Reconstitution: | Reconstitute in 250µl sterile distilled water. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Handling: | After reconstitution, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B: K. Fettucciari, et al.; Cell. Mol. Life Sci.
79, 442 (2022),
Abstract;
Galvanic current activates the NLRP3 inflammasome to promote Type I collagen production in tendon: A.P. Franch, et al.; Elife
11, e73675 (2022),
Abstract;
Enteric glial cells are susceptible to Clostridium difficile toxin B: K. Fettucciari, et al.; Cell. Mol. Life Sci.
74, 1527 (2017),
Abstract;
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