Novel inhibitor of NAD(P)H oxidase (Nox). In vascular smooth muscle cells VAS-2870 completely abolished PDGF-mediated NAD(P)H oxidase activation and ROS production (100% at 10µM). It inhibits PDGF-induced chemotaxis but not proliferation of VSMC. In mouse embryonic stem cells VAS-2870 blocked PDGF-BB-induced vasculogenesis. OxLDL-induced ROS formation in human endothelial cells was completely inhibited by VAS-2870 (100% at 5µM). VAS-2870 inhibits PMS-induced oxidative burst in HL-60 cells (IC50=2µM).
Product Details
Alternative Name: | 3-Benzyl-7-(2-benzoxazolyl)thio-1,2,3-triazolo(4,5-d)pyrimidine |
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Formula: | C18H12N6OS |
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MW: | 360.4 |
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CAS: | 722456-31-7 |
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Purity: | ≥98% (HPLC) |
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Identity: | Determined by NMR. |
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Appearance: | White to off-white solid. |
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Solubility: | Soluble in DMSO (up to 10mg/ml) or DMF (up to 25mg/ml). |
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Shipping: | Ambient Temperature |
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Long Term Storage: | Ambient |
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Use/Stability: | Store solutions at -20°C for up to 3 months. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
VAS2870 and VAS3947 attenuate platelet activation and thrombus formation via a NOX-independent pathway downstream of PKC: W.J. Lu, et al.; Sci. Rep.
11, 18852 (2019),
Abstract;
Full Text
Detrimental role of pericyte Nox4 in the acute phase of brain ischemia: A. Nishimura, et al.; J. Cereb. Blood Flow Metab.
36, 1143 (2016),
Application(s): Cell Culture,
Abstract;
Full Text
Hydrogen peroxide (H2O2) controls axon pathfinding during zebrafish development: C. Gauron, et al.; Dev. Biol.
414, 133 (2016),
Application(s): Zebrafish embryos were incubated in VAS-2870 (Nox-i) (100 nM),
Abstract;
Activation of PPARδ prevents endothelial dysfunction induced by overexpression of amyloid-β precursor protein: L.V. d'Uscio, et al.; Cardiovasc. Res.
96, 504 (2012),
Application(s): Western Blot of mouse aorta lysates,
Abstract;
Full Text
H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling: J.F. Wooley, et al.; PLoS One
7, e34050 (2012),
Abstract;
Full Text
Platelet-derived growth factor BB stimulates vasculogenesis of embryonic stem cell-derived endothelial cells by calcium-mediated generation of reactive oxygen species: S. Lange, et al.; Cardiovasc. Res.
81, 159 (2009),
Abstract;
Novel Nox inhibitor of oxLDL-induced reactive oxygen species formation in human endothelial cells: C. Stielow, et al.; BBRC
344, 200 (2006),
Abstract;
Novel Nox inhibitor VAS2870 attenuates PDGF-dependent smooth muscle cell chemotaxis, but not proliferation: H. ten Freyhaus, et al.; Cardiovasc. Res.
71, 331 (2006),
Abstract;