Replaces Prod. #: ALX-270-095
DRB is a potent and specific inhibitor of casein kinase II (CKII, IC50~6 µM). It has been used to inhibit RNA polymerase II mediated-transcription which may be dependent on CKII and its interaction with ATF-1.
Product Details
| Alternative Name: | DRB, 5,6-Dichlorobenzimidazole riboside |
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| Formula: | C12H12Cl2N2O4 |
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| MW: | 319.1 |
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| CAS: | 53-85-0 |
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| Purity: | ≥97% (TLC) |
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| Identity: | Determined by 1H-NMR. |
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| Appearance: | White solid. |
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| Solubility: | Soluble in DMSO (20mg/ml), 100% ethanol, and dimethyl formamide (100mM). |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Increased 14-3-3 phosphorylation observed in Parkinson's disease reduces neuroprotective potential of 14-3-3 proteins: S.R. Slone, et al.; Neurobiol. Dis.
79, 1 (2015),
Application(s): Cell Culture,
Abstract;
Histone hyperacetylation during SV40 transcription is regulated by p300 and RNA polymerase II translocation: L. Balakrishnan & B. Milavetz; J. Mol Biol.
371, 1022 (2007),
Abstract;
Full Text
RNA synthesis block by 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) triggers p53-dependent apoptosis in human colon carcinoma cells: R.H. te Poele, et al.; Oncogene
18, 5765 (1999),
Abstract;
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Copurification of casein kinase II with transcription factor ATF/E4TF3: T. Wada, et al.; Nucleic Acids Res.
24, 876 (1996),
Abstract;
Inhibition of germinal vesicle breakdown in bovine oocytes by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB): C.E. Farin & L. Yang; Mol. Reprod. Dev.
37, 284 (1994),
Abstract;
Kinetics of inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole on calf thymus casein kinase II: R.O. Zandomeni; Biochem. J.
262, 469 (1989),
Abstract;
Casein kinase type II is involved in the inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole of specific RNA polymerase II transcription: R. Zandomeni, et al.; J. Biol. Chem.
261, 3414 (1986),
Abstract;
Mechanism of action of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. II. A resistant human cell mutant with an altered transcriptional machinery: B. Mittleman, et al.; J. Mol. Biol.
165, 461 (1983),
Abstract;
Mechanism of action of DRB. III. Effect on specific in vitro initiation of transcription: R. Zandomeni, et al. ; J. Mol. Biol.
167, 561 (1983),
Abstract;
Halobenzimidazole ribosides and RNA synthesis of cells and viruses: I. Tamm & P.B. Sehgal; Adv. Virus Res.
22, 187 (1978),
Abstract;
