NOW INCLUDES BOTH COLORIMETRIC AND FLUOROGENIC SUBSTRATES! This kit is a complete assay system to measure protease activity of recombinant caspase-1. Cleavage of a tetrapeptide substrate is monitored colorimetrically at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm). Assays are performed in a convenient 96-well plate format.
Product Details
| Applications: | Colorimetric detection, Fluorescent detection, HTS
Activity assay
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Contents: | Caspase-1 enzyme (human) (recombinant), Ac-YVAD-pNA colorimetric substrate, Ac-YVAD-AMC fluorogenic substrate, calibration standards, aldehyde inhibitor, assay buffer, 1/2-volume 96-microwell plate, and instruction booklet. |
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| Technical Info/Product Notes: | Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays |
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| UniProt ID: | P29466 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
The Ayurvedic plant Bacopa monnieri inhibits inflammatory pathways in the brain: M.D. Nemetchek, et al.; J. Ethnopharmacol.
8741, 30495 (2016),
Application(s): Enzyme inhibition assays; bacopa extracts,
Abstract;
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