A QUANTIZYME® Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OMNIMMP® fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-13, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet.
Product Details
Alternative Name: | Collagenase-3 |
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Applications: | Fluorescent detection, HTS Activity assay
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Shipping: | Dry Ice |
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Long Term Storage: | -80°C |
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Contents: | MMP-13 enzyme (human) (recombinant), substrate, calibration standard, inhibitor, assay buffer, 96-well white microplate. |
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UniProt ID: | P45452 |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Conditional activation of β‐catenin signaling in mice leads to severe defects in intervertebral disc tissue: M. Wang, et al.; Arthritis Rheum.
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Application(s): Inhibition assay,
Abstract;
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