Matrix Metalloproteinase-9 (MMP-9) fluorometric drug discovery kit



BML-AK411-0001	96 wells	654.00 USD
Do you need bulk/larger quantities?

A QUANTIZYME^® Assay System. The MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OMNIMMP^® fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-9, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH)³, and a detailed instruction booklet.

Product Details

Alternative Name:	Gelatinase B, 92kDa Type IV collagenase

Applications:	Fluorescent detection, HTS Activity assay

Shipping:	Dry Ice

Long Term Storage:	-80°C

Contents:	MMP-9 enzyme (human) (recombinant), substrate, calibration standard, inhibitor, assay buffer, 96-well white microplate.

Technical Info/Product Notes:	Application Note: Automated Monitoring of Protein Expression and Metastatic Cell Migration Using Magnetic 3D Bioprinted Colorectal Cancer Cells

UniProt ID:	P14780

Regulatory Status:	RUO - Research Use Only

Product Literature References

Elevated Neutrophil Elastase in Tears of Ocular Graft-Versus-Host Disease Patients: S.N. Arafat, et al.; Am. J. Ophthalmol. 176, 46 (2017), Abstract;

Tear Matrix Metalloproteinases and Myeloperoxidase Levels in Patients With Boston Keratoprosthesis Type I: M.C. Robert, et al.; Cornea 35, 1008 (2016), Abstract; Full Text

Neutrophil collagenase, gelatinase, and myeloperoxidase in tears of patients with stevens-johnson syndrome and ocular cicatricial pemphigoid: S.N. Arafat, et al.; Ophthalmology 121, 79 (2014), Abstract; Full Text

Targeting androgen receptor/Src complex impairs the aggressive phenotype of human fibrosarcoma cells: G. Castoria, et al.; PLoS One 8, e76899 (2013), Abstract; Full Text

Interplay between steroid receptors and neoplastic progression in sarcoma tumors: A. Fiorelli, et al.; J. Cell. Physiol. 226, 2997 (2011), Abstract;

Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue: J.S. Lee, et al.; Exp. Dermatol. 19, 1060 (2010), Abstract;

Related Products

	Matrix Metalloproteinase-9 (MMP-9) colorimetric drug discovery kit
	Colorimetric detection, HTS, Activity assay \| Print as PDF

	BML-AK410-0001	96 wells	654.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase-9 (MMP-9) fluorometric drug discovery kit, RED
	Fluorescent detection, HTS, Activity assay \| Print as PDF

	BML-AK306-0001	96 wells	714.00 USD
Do you need bulk/larger quantities?

	Matrix metalloproteinase (MMP) multipack-1
	FUNC, HTS \| Print as PDF

	BML-AK013-0001	1 Kit	899.00 USD
Do you need bulk/larger quantities?

	MMP-9 (catalytic domain) (human), (recombinant, E. coli)
	Produced in E. coli. Active recombinant matrix metalloproteinase-9 (MMP-9, gelatinase B, 92 kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Phe¹⁰⁷-Pro⁴⁴⁹ (NM_004994), which comprises the catalytic/fibronectin domain of human MMP-9, with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-9 which lacks the C-terminal hemopexin domain. Activity toward its targets, such as gelatin, casein, or peptide substrates, is unaffected., ≥95% (SDS-PAGE) \| Print as PDF

	BML-SE360-0010	10 µg	506.00 USD
Do you need bulk/larger quantities?