The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol. Cytochrome c released from mitochondria into cytosol is determined by Western blot using the cytochrome c antibody provided in the kit. The procedure is simple, straightforward and requires no ultracentrifugation.
Product Details
| Application Notes: | For the detection of cytochrome c translocation from mitochondria into cytosol during apoptosis. |
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| Quantity: | 100 tests. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Contents: | 10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 110µl DTT (1M), 1 vial 500X Protease Inhibitor Cocktail, 0.5ml Cytochrome c Antibody (0.2mg/ml). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
FTY720 inhibits tumor growth and enhances the tumor‐suppressive effect of topotecan in neuroblastoma by interfering with the sphingolipid signaling pathway: M. Li, et al.; Pediatr. Blood Cancer
60, 1418 (2013),
Abstract;
Full Text
Pseudolaric acid B induces apoptosis in U937 human leukemia cells via caspase-9 mediated activation of the mitochondrial death pathway: J. Wang, et al.; Mol. Med. Rep.
8, 787 (2013),
Abstract;
