EGFP (enhanced green fluorescent protein) has emerged as an ideal fluorochrome for FACS analysis and fluorescent microscopy as it offers a more intense signal than wild-type GFP. It has Ex(max): 488nm and Em(max): 530nm. The green signal produced makes EGFP ideal for dual staining with propidium iodide (PI).
Figure: Detection of apoptosis using Annexin V-EGFP Apoptosis Detection Kit (Prod. No.
ALX-850-253).
Method: Apoptosis was induced in Jurkat cells by campothecin (2µM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 min. acc. to kit instructions. Results were analyzed by flow cytometry."
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Product Details
| Applications: | Flow Cytometry, Fluorescence microscopy
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| Application Notes: | For the detection of apoptotic cells by flow cytometry. |
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| Handling: | Protect from light. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | Annexin V-EGFP, propidium iodide solution and binding buffer.
An assay protocol is included. |
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| Regulatory Status: | RUO - Research Use Only |
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