Caspase (active) (recombinant) set III



ALX-850-243-KI01	1 Set	Inquire for pricing
Do you need bulk/larger quantities?

Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays.

Product Details

Recommended Dilutions/Conditions:	We recommend using 1U per assay for analyzing caspase activity.

Specific Activity:	One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8, LEHD-pNA for caspase-9, and IETD-pNA for caspase-10) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Purity:	All components ≥90% (SDS-PAGE)

Contents:	Contains 25 units each of the following caspases: - Caspase-1 (active) (human) (recombinant), yellow cap - Caspase-2 (active) (human) (recombinant), orange cap - Caspase-3 (active) (human) (recombinant), red cap - Caspase-6 (active) (human) (recombinant), violet cap - Caspase-7 (active) (human) (recombinant), red cap - Caspase-8 (active) (human) (recombinant), green cap - Caspase-9 (active) (human) (recombinant), blue cap - Caspase-10 (active) (human) (recombinant), amber cap

Formulation:	Lyophilized.

Reconstitution:	Reconstitute to 0.1-1U/µl in PBS.

Shipping:	Dry Ice

Long Term Storage:	-80°C

Source:	Produced in E. coli.

Regulatory Status:	RUO - Research Use Only

Product Literature References

Partial rescue of some features of Huntington Disease in the genetic absence of caspase-6 in YAC128 mice: B.K. Wong, et al.; Neurobiol. Dis. 76C, 24 (2015), Application(s): Cell Culture, Abstract;

Related Products

	Caspase-8 (human), (recombinant) (active)
	Produced in E. coli. Contains an N-terminal His-tag., ≥95% (SDS-PAGE) \| Print as PDF

	ALX-201-062-U025	25 U	373.00 USD

	ALX-201-062-U100	100 U	634.00 USD
Do you need bulk/larger quantities?

	Caspase-7 (human), (recombinant) (active)
	Produced in E. coli. Contains a C-terminal His-tag., ≥90% (SDS-PAGE) \| Print as PDF

	ALX-201-061-U100	100 U	Inquire for pricing

	ALX-201-061-U025	25 U	Inquire for pricing
Do you need bulk/larger quantities?

	Caspase-6 (human), (recombinant) (active)
	Produced in E. coli. Contains an N-terminal His-tag., ≥95% (SDS-PAGE) \| Print as PDF

	ALX-201-060-U025	25 U	351.00 USD

	ALX-201-060-U100	100 U	619.00 USD
Do you need bulk/larger quantities?

	Caspase-3 (human), (recombinant) (active)
	Highly active caspase essential for apoptosis
	Produced in E. coli. Contains an N-terminal His-tag. \| Print as PDF

	ALX-201-059-U025	25 U	199.00 USD

	ALX-201-059-U100	100 U	455.00 USD
Do you need bulk/larger quantities?

	Caspase-2 (human), (recombinant) (active)
	Produced in E. coli. Contains an N-terminal His-tag., ≥95% (SDS-PAGE) \| Print as PDF

	ALX-201-057-U025	25 U	341.00 USD

	ALX-201-057-U100	100 U	614.00 USD
Do you need bulk/larger quantities?

	Caspase-1 (human), (recombinant) (active)
	Produced in E. coli. Contains an N-terminal His-tag. \| Print as PDF

	ALX-201-056-U025	25 U	392.00 USD

	ALX-201-056-U100	100 U	658.00 USD
Do you need bulk/larger quantities?