Product Details
| Alternative Name: | HLA Class I |
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| Clone: | W6/32 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Membrane of human tonsil cells. |
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| Species reactivity: | Human
Bovine, Cat, Monkey
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| Specificity: | Recognizes virtually all nucleated human cells. Suitable as positive control for HLA tissue typing and crossmatching. |
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| Crossreactivity: | Does not cross-react with rabbit MHC class I. |
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| Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB, FUNC
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| Recommended Dilutions/Conditions: | Western Blot (non-reducing)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.4. 15mM sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Scientific Background: | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Expression of HLA-G in human cornea, an immune-privileged tissue: M. Le Discorde, et al.; Hum. Immunol.
64, 1039 (2003),
Abstract;
HLA-B27 misfolding is associated with aberrant intermolecular disulfide bond formation (dimerization) in the endoplasmic reticulum: N.S. Dangoria, et al.; J. Biol. Chem.
277, 23459 (2002),
Abstract;
Full Text
The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex: T.M. Tran, et al.; Immunogenetics
53, 440 (2001),
Abstract;
Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody: J.J. Ladasky, et al.; Immunogenetics
49, 312 (1999),
Abstract;
Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin: M.J. Shields and R.K. Ribaudo; Tissue Antigens
51, 567 (1998),
Abstract;
Major histocompatibility complex class I molecule expression is normal on peripheral blood lymphocytes from patients with insulin-dependent diabetes mellitus: W. Hao, et al.; J. Clin. Invest.
98, 1613 (1996),
Abstract;
Full Text
Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species: C.N. Jacobsen, et al.; Vet. Immunol. Immunopathol.
39, 461 (1993),
Abstract;
Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast: P.L. Stern, et al.; J. Immunol.
138, 1088 (1987),
Abstract;
Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin: F. Kievits and P. Ivanyi; Hum. Immunol.
50, 115 (1987),
Abstract;
A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes: J.J. Neefjes, et al.; Immunogenetics
23, 341 (1986),
Abstract;
Quantitative analysis of platelet surface HLA by W6/32 anti-HLA monoclonal antibody: K.J. Kao, et al.; Blood
68, 627 (1986),
Abstract;
Full Text
Human chromosome 11 carries at least four genes controlling expression of cell-surface antigens: A. Tunnacliffe, et al.; Somatic Cell Genet.
9, 629 (1983),
Abstract;
Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies: F.M. Brodsky and P. Parham; Immunogenetics
15, 151 (1982),
Abstract;
The gene, MIC4, which controls expression of the antigen defined by monoclonal antibody F10.44.2, is on human chromosome 11: P.N. Goodfellow, et al.; Eur. J. Immunol.
12, 659 (1982),
Abstract;
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis: C.J. Barnstable, et al.; Cell
14, 9 (1978),
Abstract;
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