Product Details
| Alternative Name: | β2 integrin |
| |
| Clone: | MEM-48 |
| |
| Host: | Mouse |
| |
| Isotype: | IgG1 |
| |
| Immunogen: | Leukocytes from a patient suffering from a LGL-type leukemia. |
| |
| UniProt ID: | P05107 |
| |
| Species reactivity: | Human
|
| |
| Specificity: | Recognizes residues 534-546 in cysteine-rich repeat 3 of CD18 (β2 integrin). |
| |
| Crossreactivity: | Cross-reacts with all three leukocyte integrins. |
| |
| Applications: | Flow Cytometry, IHC (FS), IP, WB, FUNC
|
| |
| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections: recommended dilution 10µg/ml, positive tissue: spleen, microglia; frozen sections)
Western Blot (Recommended dilution: 2µg/ml in buffer containing laurylmaltoside, 2x non-reducing SDS; SDS-PAGE (6% separating gel, 4% stacking gel)
positive control: Kg-1a human leukemia cell lysate)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Functional Application: induces high-affinity LFA-1 conformation in leukocytes |
| |
| Purity Detail: | Protein A-affinity purified. |
| |
| Formulation: | Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
| |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Shipping: | Blue Ice |
| |
| Short Term Storage: | +4°C |
| |
| Long Term Storage: | -20°C |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Regulation of CD18 expression on neutrophils in response to fluid shear stress: S. Fukuda and G.W. Schmid-Schonbein; PNAS
100, 13152 (2003),
Abstract;
Full Text
A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins: K. Drbal, et al.; Immunobiology
203, 687 (2001),
Abstract;
A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells: K. Drbal, et al.; Blood
98, 1561 (2001),
Abstract;
Human leukocytes contain a large pool of free forms of CD18: K. Drbal; BBRC
275, 295 (2000),
Abstract;
Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis: L. Ottonello, et al.; J. Leukoc. Biol.
68, 662 (2000),
Abstract;
Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins: L. Ottonello, et al.; Blood
93, 3505 (1999),
Abstract;
Full Text
The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases: T. Cinek and V. Horejsi; J. Immunol.
149, 2262 (1992),
Abstract;
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein: V. Bazil, et al.; Folia Biol. (Praha)
36, 41 (1990),
Abstract;
