Product Details
| Alternative Name: | CD95, APO-1, TNFRSF 6 |
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| Clone: | 2R2 |
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| Host: | Mouse |
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| Isotype: | IgG3 |
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| Immunogen: | Mouse L cells stably overexpressing human Fas (CD95; APO-1). |
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| UniProt ID: | P25445 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Applications: | Flow Cytometry, FUNC
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| Recommended Dilutions/Conditions: | Flow Cytometry, Functional Application (induces apoptosis - no cross-linking required). , - Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation., Recommended working dilutions are 1:200-1:1,000. |
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| Application Notes: | Flow Cytometry
Functional Application (induces apoptosis - no cross-linking required).
- Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation.
Recommended working dilutions are 1:200-1:1000. |
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| Purity Detail: | Protein A-affinity purified. |
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| Formulation: | Liquid. In PBS containing 1% BSA. Contains no preservatives. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of Fas surface expression by FACS analysis.
Method: Normal L cells (open histogram) and cells stably overexpressing human Fas (filled histogram) were incubated with anti-Fas 2R2 and analyzed by indirect immunofluorescence using PE-labeled secondary antibodies. No immunstaining was obtained with an isotype matched IgG control.
Figure: Induction of apoptosis in anti-Fas 2R2 stimulated Jurkat T-cells.
Method: (A) Dose response: Jurkat cells (1 x 105) were treated with agonistic anti-Fas 2R2 antibodies at the indicated concentrations for 24 h. Induction of apoptosis was assessed by PI staining of hypodiploid nuclei and subsequent flow cytometry.
(B) Time course of apoptosis: Jurkat cells (1 x 105) were treated with 1 µg/ml anti-Fas 2R2. After the indicated time points apoptosis was determined by FACS measurement of hypodiploid DNA.
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Product Literature References
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol.
30, 1769 (2000),
Abstract;
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood
93, 3053 (1999),
Abstract;
Full Text
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med.
188, 979 (1998),
Abstract;
Full Text
