Product Details
| Alternative Name: | Leucine-rich repeat-containing protein 32, GARP, Garpin, Glycoprotein A repetitions predominant |
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| Clone: | Plato-1 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Recombinant human LRRC32 (aa 20-627). Detects a band of ~100kDa (Fc Fusion ALX-522-117) by Western blot. Native protein is ~72kDa. |
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| UniProt ID: | Q14392 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human, Mouse
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| Applications: | ELISA, IP, WB
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| Recommended Dilutions/Conditions: | Immunoprecipitation (1:200)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Cited applications include flow cytometry. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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| Handling: | For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Protect from light. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | LRRC32 (leucine rich repeat containing 32; also known as GARP or Garpin; Glycoprotein A repetitions predominant) is a glycoprotein expressed on the cell surface of megakaryocytes, platelets and activated regulatory T (Treg) cells. |
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| Technical Info/Product Notes: | 1 test means: 1µl of MAb is used to stain 500’000 cells in a sample volume of 50µl. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Pancreatic cancer cells render tumor-associated macrophages metabolically reprogrammed by a GARP and DNA methylation-mediated mechanism: M. Zhang, et al.; Signal Transduct. Target Ther.
6, 366 (2021),
Abstract;
Blocking immunosuppression by human Tregs in vivo with antibodies targeting integrin αVβ8: J. Stockis, et al.; PNAS
114, E10161 (2017),
Abstract;
A key role of GARP in the immune suppressive tumor microenvironment: S.A. Hahn, et al.; Oncotarget
7, 42996 (2016),
Application(s): Western blot,
Abstract;
Full Text
Surface Expression of TGFβ Docking Receptor GARP Promotes Oncogenesis and Immune Tolerance in Breast Cancer: A. Metelli, et al.; Cancer Res.
76, 7106 (2016),
Abstract;
Full Text
Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor β1 (TGF-β1) Production in Human Regulatory T Cells: C. Huygens, et al.; J. Biol. Chem.
290, 20105 (2015),
Abstract;
Full Text
Feline glycoprotein A repetitions predominant anchors transforming growth factor beta on the surface of activated CD4(+)CD25(+) regulatory T cells and mediates AIDS lentivirus-induced T cell immunodeficiency: M.M. Miller, et al.; AIDS Res. Hum. Retroviruses
29, 641 (2013),
Application(s): Flow cytometry,
Abstract;
Full Text
GARP is regulated by miRNAs and controls latent TGF-β1 production by human regulatory T cells: E. Gauthy, et al.; PLoS One
8, e76186 (2013),
Abstract;
Full Text
Interleukin-21 (IL-21) synergizes with IL-2 to enhance T-cell receptor-induced human T-cell proliferation and counteracts IL-2/transforming growth factor-β-induced regulatory T-cell development: A. Battaglia, et al.; Immunology
139, 109 (2013),
Application(s): Flow cytometry,
Abstract;
Full Text
CD4(+)CD25(+)Foxp3(+)IFNγ(+) Treg are immunosuppressive in vitro and increase with intensity of the alloresponse in pretransplant MLC: V. Daniel, et al.; Transpl. Immunol.
27, 114 (2012),
Application(s): Flow cytometry,
Abstract;
Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells: R. Wang, et al.; PNAS
106, 13439 (2009),
Abstract;
GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells: D.Q. Tran, et al.; PNAS
106, 13445 (2009),
Abstract;
Therapeutic potential of FOXP3+ regulatory T cells and their interactions with dendritic cells: D.Q. Tran & E.M. Shevach; Hum. Immunol.
70, 294 (2009),
Abstract;
General Literature References
Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression: R. Wang, et al.; PLoS ONE
3, e2705 (2008),
Abstract;
Full Text
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