Product Details
Alternative Name: | Bcl-2-like protein 13, Mil1 protein |
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Clone: | Rocky-2 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant human Bcl-rambo. |
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UniProt ID: | Q9BXK5 |
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Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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Species reactivity: | Human
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Specificity: | Recognizes the C-terminus of human Bcl-rambo (aa 224-459). |
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Applications: | ICC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:300) Immunoprecipitation (1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects endogenous protein by ICC, IP and WB. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Immunofluorescence microscopy showing the detection of endogenous Bcl-rambo in HELA cells with the MAb to Bcl-rambo (human) (CT) (Rocky-2) (Prod. No.
ALX-804-821).
Method: Following fixation with 2% paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) (Prod. No.
ALX-804-821) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection."
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General Literature References
Bcl-rambo beta, a special splicing variant with an insertion of an Alu-like cassette, promotes etoposide- and Taxol-induced cell death: P. Yi, et al.; FEBS Lett.
534, 61 (2003),
Abstract;
Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension: T. Kataoka, et al.; J. Biol. Chem.
276, 19548 (2001),
Abstract;
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