| ALX-804-588-C100 | 100 µg | 481.00 USD |  |
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| Alternative Name: | Heat shock protein 27 |
| Clone: | 5B9 |
| Host: | Mouse |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide corresponding to aa 79-85 (R79QLpSSGV85) of human HSP27 (heat shock protein 27) phosphorylated at Ser82 conjugated to KLH. |
| UniProt ID: | P04792 |
| GenBank ID: | L39370 |
| Species reactivity: | Human, Mouse, Rat Dog |
| Specificity: | Recognizes HSP27 phosphorylated at Ser82. |
| Applications: | IHC, WB |
| Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Positive Control: | Included. (Prod. No. ALX-840-031) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Upon reconstitution, stable at -80°C for up to 1 year, at +4°C for up to 3 months. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Technical Info/Product Notes: | Replacement for ADI-905-642 |
| Regulatory Status: | RUO - Research Use Only |
