Product Details
| Alternative Name: | Bcl-2-modifying factor |
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| Clone: | 17A9 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 (D13VFQSEDGEPGTQPG27) and aa 112-126 (A112GSPLGEQPPEGQFL126) of mouse Bmf.
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| UniProt ID: | Q91ZE9 |
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| Species reactivity: | Mouse, Rat
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| Crossreactivity: | Does not cross-react with human Bmf. |
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| Applications: | ELISA, Flow Cytometry, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. |
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| Formulation: | Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: A) Detection of overexpressed Bmf in 293T cells transiently transfected with mouse Bmf by Western blot using MAb to Bmf (17A9) (Prod. No.
ALX-804-508).
B) Detection of endogenous Bmf by Western blot using MAb to Bmf (17A9) (Prod. No.
ALX-804-508) in mouse spleen, lymph node and thymus as two bands of ~25 and ~30kDa, but not in these tissues derived from two knock-out Bmf strains del 372 and del 376."
Figure: C) Detection of mouse and rat Bmf by Western blot using MAb to Bmf (17A9) (Prod. No.
ALX-804-508).
D) Immunoprecipitation of endogenous Bmf from mouse spleen cells using MAb to Bmf (17A9) (Prod. No.
ALX-804-508)."
Figure: Flow cytometry data of 293T cells transiently transfected with EE-tagged Bmf, fixed with 1% PFA, permeabilised with saponin and stained intracellularly with Bmf (mouse/rat), mAb (17A9) (Prod. No. ALX-804-508) or positive control anti-EE.
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Product Literature References
BH3-only protein Bmf mediates apoptosis upon inhibition of CAP-dependent protein synthesis: F. Grespi, et al.; Cell Death Differ.
17, 1672 (2010),
Abstract;
Full Text
BID, BIM, and PUMA are essential for activation of the BAX- and BAK-dependent cell death program: D. Ren, et al.; Science
330, 1390 (2010),
Abstract;
Full Text
The role of BH3-only protein Bim extends beyond inhibiting Bcl-2–like prosurvival proteins.: D. Mérino, et al.; J. Cell. Biol.
186, 355 (2009),
Abstract;
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med.
205, 641 (2008),
Abstract;
Full Text
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis: S. Ying, et al.; Infect. Immun.
73, 1399 (2005),
Abstract;
Full Text
General Literature References
Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science
293, 1829 (2001), Methodology,
Abstract;
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques
25, 824 (1998), Methodology,
Abstract;
