Product Details
| Alternative Name: | Activating transcription factor 6 |
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| Clone: | 70B1413.1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Partial human ATF6 (activating transcription factor 6) containing aa 1-273. |
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| UniProt ID: | P18850 |
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| Species reactivity: | Human, Mouse, Rat
Hamster, Rabbit
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| Applications: | ICC, IHC (PS), IP, WB
ChIP
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| Recommended Dilutions/Conditions: | Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands of ~90kDa (full length ATF6) and ~50-70kDa (cleaved forms of ATF6) by Western blot. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. |
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| Use/Stability: | Stable for 6 months when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Western blot analysis of ATF6 on 15µg of (A) Jurkat and (B) NIH3T3 cell lysates using MAb to ATF6 (70B1413.1) (Prod. No.
ALX-804-381). A band of ~90kDa was detected."
Figure 2: Western blot analysis of ATF6 using MAb to ATF6 (70B1413.1) (Prod. No.
ALX-804-381).
Method: Western blots were probed with 4µg/ml of MAb to ATF6 (70B1413.1), followed by an HRP-conjugated second step and visualized with PicoTect Western Blot Chemiluminescence Substrate. Film was exposed for 1 min. The top arrow corresponds to the ~90kDa form of ATF6 described as full-length in the literature.
Lane 1: 293 cells transfected with full-length ATF6.
Lane 2: 293 cells transfected with partial length ATF6 (aa 1-373).
Lane 3: Untransfected 293 cells. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002) [1]."
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Product Literature References
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Response of myeloma to the proteasome inhibitor bortezomib is correlated with the unfolded protein response regulator XBP-1: S.C. Ling, et al.; Haematologica
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Eif-2a protects brainstem motoneurons in a murine model of sleep apnea: Y. Zhu, et al.; J. Neurosci.
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Acetaminophen induces ER dependent signaling in mouse liver: G. Nagy, et al.; Arch. Biochem. Biophys.
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3,3’-diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5: M. Abdelrahim, et al.; Carcinogenesis
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Cytoprotective gene bi-1 is required for intrinsic protection from endoplasmic reticulum stress and ischemia-reperfusion injury: B. Bailly-Maitre, et al.; PNAS
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Endoplasmic reticulum stress and neurodegeneration in rats neonatally infected with borna disease virus: B.L. Williams and W.I. Lipkin; J. Virol.
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Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress: C.Y. Yu, et al.; J. Virol.
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Glia-specific activation of all pathways of the unfolded protein response in vanishing white matter disease: B. van Kollenburg, et al.; J. Neuropathol. Exp. Neurol.
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Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase: A.C. Nyborg, et al.; FASEB J.
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Intrinsic capacities of molecular sensors of the unfolded protein response to sense alternate forms of endoplasmic reticulum stress: J.B. DuRose, et al.; Mol. Biol. Cell
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Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system: A.J. Okumura, et al.; J. Biol. Chem.
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Nitric oxide-induced endoplasmic reticulum stress activates the expression of cargo receptor proteins and alters the glycoprotein transport to the Golgi complex: M. Renna, et al.; Int. J. Biochem. Cell Biol.
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Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model: H. Kikuchi, et al.; PNAS
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The unfolded protein response modulates toxicity of the expanded glutamine androgen receptor: M. Thomas, et al.; J. Biol. Chem.
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A signal peptide peptidase (SPP) reporter activity assay based on the cleavage of type II membrane protein substrates provides further evidence for an inverted orientation of the SPP active site relative to presenilin: A.C. Nyborg, et al.; J. Biol. Chem.
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Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response: M. Hong, et al.; J. Biol. Chem.
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Kaposi’s sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile: R.G. Jenner, et al.; PNAS
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Requirement of the p38 mitogen-activated protein kinase signalling pathway for the induction of the 78 kDa glucose-regulated protein/immunoglobulin heavy-chain binding protein by azetidine stress: activating transcription factor 6 as a target for stress-i: S. Luo and A.S. Lee; Biochem. J.
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