Product Details
| Alternative Name: | BCL2L11 |
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| Clone: | 14A8 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant mouse BimS. |
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| UniProt ID: | O54918 |
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| Species reactivity: | Human, Mouse
Monkey
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| Specificity: | Recognizes BimL, BimEL and BimS. |
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| Applications: | Flow Cytometry, ICC, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (0.5-1µg/ml; overexpressing cell lines)
Immunocytochemistry (1-5µg/ml)
Immunohistochemistry (paraffin sections, 1-5µg/ml)
Immunoprecipitation (1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 10mM sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | As Bim expression is generally low in non-transfected cell lines, please take care when choosing positive controls. Examples of cell lines which express detectable levels of Bim include: B6.2.16.BW2 or KO52.DA20 (T cell lymphomas) or ABL5.8.1 (B cell lymphoma) or MCF-7s. It is generally necessary to use a high amount of lysate (e.g. 1 x 10^7 cells). When using tissue samples, it is generally necessary to use 1-5mg of total protein from an appropriate tissue source. |
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| Regulatory Status: | RUO - Research Use Only |
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-n.gif "BimL monoclonal antibody (5E5) Western blot")
Figures: IP/Western blot analysis of Bim expression in mouse (A) and human (C) cell lines. Bim proteins were immunoprecipitated from lysates of 10
7 cells with anti-Bim
S/EL/L MAb (clone 14A8) (Prod. No.
ALX-804-351) and anti-Bim
L MAb (clone 5E5) (Prod. No.
ALX-804-352). Western blot analysis was performed with clones 14A8 and 5E5 followed by the addition of biotinylated mouse anti-rat IgG2b antibody and HRP-conjugated mouse anti-rat IgG2a antibody as the respective secondary antibodies, and ECL detection. (B) depicts the ability of clone 14A8 to immunoprecipitate Bim
L and Bim
EL from lysates of spleen and thymus from normal mice, but not from Bim-deficient mice."
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Product Literature References
The role of BH3-only protein Bim extends beyond inhibiting Bcl-2–like prosurvival proteins.: D. Mérino, et al.; J. Cell. Biol.
186, 355 (2009),
Abstract;
Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells: Y. Zhu, et al.; PNAS
101, 7681 (2004),
Abstract;
Full Text
BH3-only Bcl-2 family member Bim is required for apoptosis of autoreactive thymocytes: P. Bouillet, et al.; Nature
415, 922 (2002),
Abstract;
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis: G.V. Putcha, et al.; Neuron
29, 615 (2001),
Abstract;
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol.
157, 449 (2000),
Abstract;
Full Text
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science
286, 1735 (1999),
Abstract;
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell
3, 287 (1999),
Abstract;
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques
25, 824 (1998),
Abstract;
