Product Details
| Alternative Name: | Apoptosis protease-activating factor 1 |
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| Clone: | 13F11 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant mouse Apaf-1 (apoptosis protease-activating factor 1) (aa 1-97) containing the N-terminal CARD domain. |
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| UniProt ID: | O88879 |
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| Species reactivity: | Mouse, Rat
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| Specificity: | Recognizes the CARD domain of Apaf-1. |
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| Crossreactivity: | Does not cross-react with human Apaf-1. |
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| Applications: | ICC, IP, WB
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figures: Species cross-reactivity of MAb to Apaf-1 (clone 13F11) (
ALX-804-349) by Western blot.
A: Lysates from 1-day-old
mouse brain (lane 1), adult
mouse brain (lane 2) and SH-SY5Y
human neuroblastoma cell line (lane 3) were probed with clone 13F11.
B: Lysates from 2-days-old
rat brain (lane 1) and adult
rat brain (lane 2) were probed with clone 13F11.
Results: 13F11 is mouse and rat specific but does not recognize human Apaf-1."
Figure: Western blot analysis with anti-Apaf-1 MAb clone 13F11 on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1- mice.
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Product Literature References
Inhibition of apoptosome formation by suppression of Hsp90beta phosphorylation in tyrosine kinase-induced leukemias: M. Kurokawa, et al.; Mol. Cell Biol.
28, 5494 (2008),
Abstract;
Full Text
Essential postmitochondrial function of p53 uncovered in DNA damage-induced apoptosis in neurons: A.E. Vaughn & M. Deshmukh; Cell Death Differ.
14, 973 (2007),
Abstract;
Full Text
Decreased apoptosome activity with neuronal differentiation sets the threshold for strict IAP regulation of apoptosis: K.M. Wright, et al.; J. Cell Biol.
167, 303 (2004),
Abstract;
Full Text
General Literature References
Apaf1 in developmental apoptosis and cancer: how many ways to die?: F. Cecconi & P. Gruss; Cell. Mol. Life Sci.
58, 1688 (2001), (Review),
Abstract;
Pro-apoptotic apoptosis protease-activating factor 1 (Apaf-1) has a cytoplasmic localization distinct from Bcl-2 or Bcl-x(L): G. Hausmann, et al.; J. Cell Biol.
149, 623 (2000),
Abstract;
Full Text
Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development: F. Cecconi, et al.; Cell
94, 727 (1998),
Abstract;
Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation: Y. Hu, et al.; PNAS
95, 4386 (1998),
Abstract;
Full Text
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell
90, 405 (1997),
Abstract;
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell
91, 479 (1997),
Abstract;
