| ALX-804-244-C100 | 100 µg | 471.00 USD |  |
||
| Do you need bulk/larger quantities? | |||||
| Alternative Name: | ODF, TRANCE, OPGL, TNFSF 11, CD254 |
| Clone: | 12A380 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant mouse RANKL (aa 1-317). |
| UniProt ID: | O35235 |
| Species reactivity: | Human, Mouse |
| Applications: | Flow Cytometry, IHC (FS), WB |
| Recommended Dilutions/Conditions: | Western Blot (4-6µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~40kDa by Western blot. |
| Positive Control: | Activated EL4 cells (mouse) or Saos-2 (human) cells can be used as positive control. |
| Purity Detail: | Protein G-affinity purified. |
| Formulation: | Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. |
| Use/Stability: | Stable for at least 6 months when stored at +4°C. |
| Shipping: | Blue Ice |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Scientific Background: | RANKL (TRANCE, OPGL, ODF) is a member of the TNF family. RANKL (receptor activator of NF-κB ligand) or TRANCE (TNF-related activation-induced cytokine receptor) has been implicated in interactions between T cells and dendritic cells. RANKL binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. RANKL is also important in T- and B-cell maturation. As OPGL (osteoprotegerin ligand) or ODF (osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. RANKL deficient mice show severe osteoporesis and complete abscence of osteoclasts as a result of lack of osteogenesis. |
| Regulatory Status: | RUO - Research Use Only |
