Product Details
| Alternative Name: | Mortalin, Heat shock protein 70 (mitochondrial), GRP75 |
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| Clone: | JG1 |
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| Host: | Mouse |
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| Isotype: | IgG3 |
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| Immunogen: | Synthetic peptide corresponding to C-terminal aa 615-633 (G615SGSSGTGEQKEDQKEEKQ633) of mouse mtHSP70 (mitochondrial heat shock protein 70). |
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| UniProt ID: | P38647 |
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| Species reactivity: | Human, Mouse
Dog, Monkey
|
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| Applications: | ICC, IP, WB
|
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:50)
Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~75kDa by Western blot. |
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| Purity Detail: | Protein A purified. |
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| Formulation: | Liquid. In PBS with 1 mg/mL BSA containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Immunolocalization of mtHSP70 in human fibroblasts using MAb to to mtHSP70 (JG1) (Prod. No.
ALX-804-077)."
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Product Literature References
Neuralized-like protein 4 (NEURL4) mediates ADP-ribosylation of mitochondrial proteins: M.D. Cardamone, et al.; J. Cell Biol.
221, e202101021 (2022),
Abstract;
Ozone Activates the Nrf2 Pathway and Improves Preservation of Explanted Adipose Tissue In Vitro: B. Cisterna, et al.; Antioxidants (Basel)
9, 989 (2020),
Abstract;
Full Text
HDAC6 maintains mitochondrial connectivity under hypoxic stress by suppressing MARCH5/MITOL dependent MFN2 degradation: H.J. Kim, et al.; Biochem. Biophys. Res. Commun.
464, 1235 (2015),
Application(s): Immunostaining,
Abstract;
Characterization of stress response in human retinal epithelial cells: V. Giansanti, et al.; J. Cell. Mol. Med.
17, 103 (2013),
Application(s): ICC on human adult retinal pigmented epithelial (ARPE-19) cells,
Abstract;
Full Text
Reversible interactions between smooth domains of the endoplasmic reticulum and mitochondria are regulated by physiological cytosolic Ca2+ levels: J.G. Goetz, et al.; J. Cell Sci.
120, 3553 (2007),
Abstract;
Full Text
Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SCO1 or SURF1: S.L. Williams, et al.; J. Biol. Chem.
279, 7462 (2004),
Abstract;
The life span determinant p66Shc localizes to mitochondria where it associates with mitochondrial heat shock protein 70 and regulates trans-membrane potential: F. Orsini, et al.; J. Biol. Chem.
279, 25689 (2004),
Abstract;
Generation and characterization of monoclonal antibodies specific for members of the mammalian 70-kDa heat shock protein family: J.M. Green, et al.; Hybridoma
14, 347 (1995),
Abstract;
