Product Details
Alternative Name: | Heat shock protein 60 (bacterial) |
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Clone: | A57-B9 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant Chlamydia trachomatis HSP60 (heat shock protein 60). |
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UniProt ID: | P0C0Z7 |
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Species reactivity: | Chlamydia
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Specificity: | Recognizes all three genera of Chlamydia HSP60. Epitope mapping studies suggest binding of aa 517-522. |
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Crossreactivity: | Minimally cross-reacts with Borrelia burgdorfi HSP60. Does not cross-react with E. coli GroEL or human HSP60. |
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Applications: | ICC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:500) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~60kDa by Western blot. |
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Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Chlamydia trachomatis inhibits apoptosis in infected cells by targeting the pro-apoptotic proteins Bax and Bak: C.W. Kontchou, et al.; Cell Death Differ.
29, 2046 (2022),
Abstract;
Infection of epithelial cells with Chlamydia trachomatis inhibits TNF-induced apoptosis at the level of receptor internalization while leaving non-apoptotic TNF-signalling intact: C. Waguia Kontchou, et al.; Cell. Microbiol.
18, 1583 (2016),
Abstract;
Monoclonal antibodies define genus-specific, species-specific and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): Specific immunodetection and purification of chlam: Y. Yuang, et al.; Infect. Immun.
60, 2288 (1992),
Abstract;