| ALX-804-061-C100 | 100 µg | 699.00 USD |  |
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| Alternative Name: | MAPK2/3, MAP kinase 2/3 |
| Clone: | 12D4 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2 conjugated to KLH. |
| UniProt ID: | P27361 (Erk1), P28482 (Erk2) |
| Species reactivity: | Human, Mouse, Rat Dog |
| Specificity: | Recognizes the pThr-Glu-pTyr motif of activated ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. |
| Crossreactivity: | Does not cross-react with activated JNK or p38. |
| Applications: | ELISA, ICC, IHC, IP, WB |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1-10µg/ml) Immunoprecipitation (1-10µg per 106 pervanadate-treated A431 or HepG2 cells) Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Positive Control: | Included. (Prod. No. ALX-840-025) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Technical Info/Product Notes: | Replacement for ADI-905-651 |
| Regulatory Status: | RUO - Research Use Only |
