Product Details
Alternative Name: | Plasma membrane calcium pump ATPase |
|
Clone: | 5F10 |
|
Host: | Mouse |
|
Isotype: | IgG2a |
|
Immunogen: | Purified human erythrocyte PMCA ATPase (plasma membrane calcium pump ATPase). |
|
UniProt ID: | Q00804 (PMCA1), Q01814 (PMCA2) |
|
Species reactivity: | Human, Mouse, Rat Amphibian, Cat, Chicken, Dog, Monkey, Rabbit, Sheep
|
|
Specificity: | Recognizes all known (currently four) isoforms of plasma membrane PMCA ATPase. Recognizes an epitope between aa 724-783 of human PMCA ATPase. |
|
Applications: | ICC, IHC (FS), IHC (PS), IP, WB
|
|
Recommended Dilutions/Conditions: | Immunohistochemistry (frozen sections, 1:500) Immunohistochemistry (paraffin sections, 1:500) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Application Notes: | Detects a band of ~140kDa by Western blot. Also detects bands at ~95kDa and ~180kDa which may represent aggregation and/or natural proteolytic products of PMCA ATPase. |
|
Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta: E.E. Champion, et al.; Placenta
24, 453 (2003),
Abstract;
Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies: A.G. Filoteo, et al.; J. Biol. Chem.
272, 23741 (1997),
Abstract;
Full Text
Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules: J.L. Borke, et al.; Am. J. Physiol.
257, F842 (1989),
Abstract;