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[pThr²⁸⁶]Ca²⁺/calmodulin-dependent protein kinase II (rat) (α subunit) monoclonal antibody (22B1)



ALX-802-009-R100	100 µl	604.00 USD
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Replaces Prod. #: BML-SA234

Product Details

Alternative Name:	CaM kinase II α subunit, CAMK2 α subunit

Clone:	22B1

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to aa 281-294 (M²⁸¹HRQET(PSO₃)VDCLKKFN²⁹⁴) of the α-subunit of mouse and rat CaM kinase II (Ca²⁺/Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr²⁸⁶).

UniProt ID:	P11798 (mouse), P11275 (rat)

Species reactivity:	Human, Mouse, Rat

Specificity:	Recognizes the phosphorylated α-subunit of CaM kinase II. This antibody does not detect the non-phosphorylated α-subunit of rat CaM kinase II nor the ~60kDa β-subunit in either phosphorylation state.

Applications:	ELISA, Flow Cytometry, ICC, IF, IP, WB

Recommended Dilutions/Conditions:	Flow Cytometry (FACS, 1μg 10⁶ cells) Immunocytochemistry (1:100) Immunofluorescence (1:100) Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Formulation:	Liquid. In PBS containing 0.05% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

Product Literature References

Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II: C.M. Atkins, et al.; J. Neurosci. 24, 5193 (2004), Abstract; Full Text

Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development: L.M. Esteban, et al.; Mol. Cell. Biol. 21, 1444 (2001), Abstract; Full Text

Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies: B.L. Patton, et al.; Mol. Biol. Cell 4, 159 (1993), Abstract;