Product Details
| Source: | Isolated from Salmonella minnesota R595 (Re mutant). Prepared by mild acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water. |
| |
| Concentration: | 1mg/ml |
| |
| Formulation: | Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water. |
| |
| Purity: | ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) |
| |
| Activity: | Activates Toll-like receptor (TLR) 4. Does not activate TLR2 as determined with splenocytes and macrophages from TLR4 deficient mice. |
| |
| Shipping: | Ambient Temperature |
| |
| Long Term Storage: | +4°C |
| |
| Handling: | Keep sterile. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
CD14 is required for MyD88-independent LPS signaling: Z. Jiang, et al.; Nat. Immunol.
6, 565 (2005),
Abstract;
IL-12 family members: differential kinetics of their TLR4-mediated induction by Salmonella Enteritidis and the impact of IL-10 in bone marrow-derived macrophages: N. Schuetze, et al.; Int. Immunol.
17, 649 (2005),
Abstract;
Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chemistry
9, 11 (1993),
Abstract;
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem.
54, 603 (1975),
Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem.
9, 245 (1969),
Abstract;
