Product Details
| Source: | Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water. |
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| Concentration: | 1mg/ml |
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| Formulation: | Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water. |
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| Purity: | ≥99.9% (regarding protein or DNA contaminants; KDO not detectable) |
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| Activity: | Activates Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | +4°C |
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| Handling: | Keep sterile. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Activation of macrophages from TLR4 wild type compared to TLR4 deficient mice by LPS and Lipid A from Enzo. Lipid A or LPS concentrations, which induced maximal activation of TLR4 wild type mouse macrophages, were also applied to TLR4 deficient mouse macrophages. 10 units of IL-6 correspond to the detection limit of the IL-6 ELISA.
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Product Literature References
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