Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism.
Product Details
| Alternative Name: | Intelectin-1, Endothelial lectin HL-1, Intestinal lactoferrin receptor, Galactofuranose-binding lectin |
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| MW: | ~40kDa |
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| Source: | Produced in HEK 293 cells. Human omentin 1 (intelectin-1) (aa 17-313) is fused at the N-terminus to a FLAG®-tag. |
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| UniProt ID: | Q8WWA0 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). |
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| Reconstitution: | Reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been identified as a visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1μg Omentin-1.
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Product Literature References
Hepatic scavenger receptor BI is associated with type 2 diabetes but unrelated to human and murine non-alcoholic fatty liver disease: L. Rein-Fischboeck, et al.; Biochem. Biophys. Res. Commun.
467, 377 (2015),
Application(s): Cell Culture,
Abstract;
Intelectin 1 suppresses the growth, invasion and metastasis of neuroblastoma cells through up-regulation of N-myc downstream regulated gene 2: D. Li, et al.; Mol. Cancer
14, 47 (2015),
Application(s): Cell Culture,
Abstract;
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Hypotensive effects of omentin-1 related to increased adiponectin and decreased interleukin-6 in intra-thoracic pericardial adipose tissue: L. Brunetti, et al.; Pharmacol. Rep.
66, 991 (2014),
Abstract;
Orexigenic effects of omentin-1 related to decreased CART and CRH gene expression and increased norepinephrine synthesis and release in the hypothalamus: L. Brunetti, et al.; Peptides
44, 66 (2013),
Abstract;
Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose: B.K. Tan, et al.; Diabetes
57, 801 (2008),
Abstract;
General Literature References
Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action: R.Z. Yang, et al.; Am. J. Physiol. Endocrinol. Metab.
290, E1253 (2006),
Abstract;
Full Text
Human homologs of the Xenopus oocyte cortical granule lectin XL35: J.K. Lee, et al.; Glycobiology
11, 65 (2001),
Abstract;
Full Text
Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall: S. Tsuji, et al.; J. Biol. Chem.
276, 23456 (2001),
Abstract;
Full Text
Molecular cloning and functional expression of a human intestinal lactoferrin receptor: Y.A. Suzuki, et al.; Biochemistry
40, 15771 (2001),
Abstract;
