Product Details
| Alternative Name: | IL-1F2, Interleukin-1β |
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| Source: | Produced in E. coli. Human IL-1β (interleukin-1β) (aa 117-270) is fused to a linker peptide (10 aa) and an N-terminal FLAG®-tag. |
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| UniProt ID: | P01584 |
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| Concentration: | 0.1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc.). |
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| Specificity: | Binds to human IL-1R (interleukin-1 receptor). |
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| Reconstitution: | Reconstitute with 100µl sterile water. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | Historical data has shown that IL-1β activates the NF-κB pathway.
FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: IL-1b activity was assessed by its ability to induce the activation of the NF-kB pathway. IkBa degradation was detected by Western blotting.
Method: Hela cells were grown to confluence in 6 well plates. IL-1b was diluted at the indicated concentrations in 500µl DMEM/Nut.Mix.F-12 10% fetal calf serum and added to the wells after being washed once with the same media. Cells were collected after 30 min and lysed. 100µg of cellular extract were separated on a SDS-PAGE and transferred on a nitrocellulose membrane. IkBa degradation was detected with a rabbit polyclonal antibody to IkBa and a goat anti-rabbit HRP-conjugated secondary antibody. Degradation of IkBa was detected at 10pg/µl IL-1b, under these conditions.
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General Literature References
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Human interleukin 1: I. Gery & J.A. Schmidt; Meth. Enzymol.
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Interleukin 1: an immunological perspective: S.K. Durum, et al.; Annu. Rev. Immunol.
3, 263 (1985),
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