Product Details
| Alternative Name: | TNFRSF 5 |
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| MW: | ~54kDa (SDS-PAGE). |
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| Source: | Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1. |
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| UniProt ID: | P25942 |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg purified protein (LAL test; Associates of CAPE COD Inc). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds human and mouse CD40L (CD154). |
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| Applications: | ELISA
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| Application Notes: | ELISA: binds to CD40L at 1-10 ng/ml.
Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100µg/ml depending on the level of CD40L surface expression. |
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| Reconstitution: | Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Jurkat T cells clone D1.1 expresses high surface levels of CD40L. Cells were immunostained at 4°C for 30 minutes with 10µg/ml of CD40:Fc (Prod. No
ALX-522-016). After washing, cells were incubated for 30 min. at 4°C with 10µg/ml goat anti-human RPE (Prod. No
ALX-211-501). After washing, cells were analyzed by Flow Cytometry. "
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Product Literature References
CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons: P. Carriba & A.M. Davies; Elife
6, e30442 (2017),
Abstract;
Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods
237, 159 (2000),
Abstract;
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