Product Details
| Alternative Name: | NGF 2.5S |
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| MW: | ~26kDa; homodimer. |
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| Source: | Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984). |
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| CAS: | 93928-24-6 |
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| UniProt ID: | P01139 |
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| Quantity: | mNGF 2.5S concentration is estimated spectroscopically from its extinction coefficient and amino acid analysis. |
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| Formulation: | Lyophilized. |
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| Purity: | ≥98% (HPLC) |
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| Biological Activity: | mNGF 2.5S was bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7 - 14 days. The neurotrophic activity is in the range of 0.5-50ng/ml. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium. |
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| Reconstitution: | Reconstitute in sterile water or DMEM at a concentration of at least 10µg/ml and store at -80°C before use. Medium with mNGF cannot be reused due to mNGF degradation. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature
368, 193 (1994), (Review),
Abstract;
Neurotrophic factors enter the clinic: M. Barinaga; Science
264, 772 (1994), (Review),
Abstract;
Neurotrophic factors: two are better than one: R. Nishi; Science
265, 1052 (1994), (Review),
Abstract;
Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J.
8, 738 (1994), (Review),
Abstract;
The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev.
18, 143 (1994), (Review),
Abstract;
Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C. R. Acad. Sci. III
316, 1158 (1993), (Review),
Abstract;
The changing scene of neurotrophic factors: H. Thoenen; TINS
14, 165 (1991), (Review),
Abstract;
Nerve growth factor: R.A. Bradshaw; Annu. Rev. Biochem.
47, 191 (1978),
Abstract;
