| ALX-260-118-M005 | 5 mg | 256.00 USD |  |
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| ALX-260-118-M010 | 10 mg | 484.00 USD | |
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| Do you need bulk/larger quantities? | |||||
| Alternative Name: | Caspase-8 substrate (fluorogenic) |
| Sequence: | Ac-Leu-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin) |
| Formula: | C31H38F3N5O12 |
| MW: | 729.7 |
| CAS: | 210345-02-1 |
| Purity: | ≥97% (HPLC) |
| Appearance: | White to off-white powder. |
| Solubility: | Soluble in DMSO. |
| Shipping: | Ambient Temperature |
| Long Term Storage: | -20°C |
| Use/Stability: | 20mM stock solution in DMSO is stable at -20°C for 6-8 months. When taking out of the freezer wait until it warms up to room temperature, then open it. |
| Handling: | Keep cool and dry. |
| Technical Info/Product Notes: | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |
| Protocol: | HEPES-Buffer (2x): 40 mM HEPES, pH 7.5, 20% glycerol, 4mM DTT. Dilute to 1x with sterile distilled water prior to use. Substrate: Prepare 20 mM stock solution in DMSO. - Induce apoptosis and prepare cell lysate or use recombinant caspase. - Prepare reaction buffer: 10µl of substrate stock solution + 1ml 1x HEPES-Buffer for each reaction. - Add an appropriate amount of cell lysate (50- 100 µl; should be titrated) or recombinant caspase to reaction buffer. - Incubate for 1 hour at 37 °C. - Measure with spectrofluorometer: 400 nm excitation wavelength, 505 nm emission wavelength. Suggested controls: Reaction mixture without substrate. Reaction mixture with non-apoptotic cell lysate. Reaction mixture with apoptotic cell lysate and caspase inhibitor. |
| Regulatory Status: | RUO - Research Use Only |
