Replaces Prod. #: BML-P433
Fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. Also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site.
Product Details
| Alternative Name: | Caspase-8 substrate (fluorogenic), Granzyme B substrate (fluorogenic) |
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| Sequence: | Ac-Ile-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin) |
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| Formula: | C31H38F3N5O12 |
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| MW: | 729.7 |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Keep cool and dry. |
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| Technical Info/Product Notes: | AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout: S. Devi, et al.; Cell Rep.
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Abstract;
Nimbolide reduces CD44 positive cell population and induces mitochondrial apoptosis in pancreatic cancer cells: S. Kumar, et al.; Cancer Lett.
413, 82 (2017),
Abstract;
Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway: S.M. Leow, et al.; Oncotarget
8, 16170 (2017),
Abstract;
Full Text
Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells: Y. Pang, et al.; Exp. Cell Res.
16, 30161 (2016),
Application(s): Fluorometric assay for caspase-8 activity,
Abstract;
Insights into the mechanism of human papillomavirus E2-induced procaspase-8 activation and cell death: N. Singh, et al.; Sci. Rep.
6, 21408 (2016),
Application(s): Caspase-8 activity assay,
Abstract;
Mechanism of neem limonoids-induced cell death in cancer: Role of oxidative phosphorylation: N. Yadav, et al.; Free Radic. Biol. Med.
90, 261 (2016),
Application(s): Quantification of apoptosis and caspase activity measurement,
Abstract;
Organ specific alteration in caspase expression and STK3 proteolysis during the aging process: M. Lessard-Beaudoin, et al.; Neurobiol. Aging
47, 50 (2016),
Application(s): Caspase activity assays,
Abstract;
Full Text
Protolichesterinic acid enhances doxorubicin-induced apoptosis in HeLa cells in vitro: F. Brisdelli, et al.; Life Sci.
158, 89 (2016),
Application(s): Caspase activity,
Abstract;
A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils: B. Geering, et al.; Blood
117, 5953 (2011),
Application(s): Caspase activity detected in human neutophils,
Abstract;
Full Text
Rapid cytochrome c release, activation of caspases 3, 6, 7 and 8 followed by Bap31 cleavage in HeLa cells treated with photodynamic therapy: D.J. Granville, et al.; FEBS Lett.
437, 5 (1998),
Abstract;
General Literature References
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem.
272, 1965 (1997),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature
371, 346 (1994),
Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol.
244, 615 (1994),
Abstract;
