Replaces Prod. #: BML-P451
Colorimetric caspase substrate. Combinatorial studies have found the WEHD sequence to be optimal for clevage of tetrapeptide substrate by caspase-1, caspase-4, caspase-5 and caspase-14. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm.
Product Details
| Alternative Name: | Caspase-1 substrate (chromogenic), Caspase-5 substrate (chromogenic) |
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| Sequence: | Ac-Trp-Glu-His-Asp-pNA (pNA=p-Nitroaniline) |
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| Formula: | C34H37N9O11 |
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| MW: | 747.7 |
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| Formulation: | Lyophilized. |
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| Peptide Content: | 70-90% |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | White to yellow powder. |
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| Solubility: | Soluble in DMSO. Dilute with buffer, pH 7.5. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Handling: | Keep cool and dry. |
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| Technical Info/Product Notes: | λmax of pNA is 400nm. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Purification and catalytic properties of human caspase family members: M. Garcia-Calvo et al.; Cell Death Differ.
6, 362 (1999),
Abstract;
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
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