Irreversible, cell permeable inhibitor of caspase-3 and -7.
Product Details
| Alternative Name: | Caspase-3 inhibitor (fluoromethylketone), Caspase-7 inhibitor (fluoromethylketone) |
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| Sequence: | Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone |
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| Formula: | C30H41FN4O12 |
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| MW: | 668.7 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (TLC) |
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| Solubility: | Soluble in DMSO (20mM) or dimethyl formamide. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for 1 year after receipt when stored at -20°C. |
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| Handling: | Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | We recommend a final concentration of 2-10µM for inhibiting caspase activity in cell culture. The optimal doses may vary for different cells and culture conditions. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Caspase-3-dependent and -independent degradation of 28 S ribosomal RNA may be involved in the inhibition of protein synthesis during apoptosis initiated by death receptor engagement: D. Nadano & T.A. Sato; J. Biol. Chem.
275, 13967 (2000),
Abstract;
Full Text
Connective tissue growth factor induces apoptosis via caspase 3 in cultured human aortic smooth muscle cells: K. Hishikawa, et al.; Eur. J. Pharmacol.
392, 19 (2000),
Abstract;
The neuroprotective effect of pituitary adenylate cyclase-activating polypeptide on cerebellar granule cells is mediated through inhibition of the CED3-related cysteine protease caspase-3/CPP32: D. Vaudry, et al.; PNAS
97, 13390 (2000),
Abstract;
Full Text
Activation of caspase-3 in the retina of transgenic rats with the rhodopsin mutation s334ter during photoreceptor degeneration: C. Liu, et al.; J. Neurosci.
19, 4778 (1999),
Abstract;
Full Text
Caspase-8 mediates caspase-3 activation and cytochrome c release during singlet oxygen-induced apoptosis of HL-60 cells: S. Zhuang, et al.; Exp. Cell Res.
250, 203 (1999),
Abstract;
Caspase inhibitor affords neuroprotection with delayed administration in a rat model of neonatal hypoxic-ischemic brain injury: Y. Cheng, et al.; J. Clin. Invest.
101, 1992 (1998),
Abstract;
Geranylgeraniol potently induces caspase-3-like activity during apoptosis in human leukemia U937 cells: Y. Masuda, et al.; BBRC
234, 641 (1997),
Abstract;
CPP32/apopain is a key interleukin 1 β converting enzyme-like protease involved in Fas-mediated apoptosis: J. Schlegel, et al.; J. Biol. Chem.
271, 1841 (1996),
Abstract;
Full Text
ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis: D.W. Nicholson; Nature Biotechnol.
14, 297 (1996),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
