Product Details
| Alternative Name: | CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, MAVS, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter |
| |
| Host: | Rabbit |
| |
| Immunogen: | Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450). |
| |
| UniProt ID: | Q7Z434 |
| |
| Source: | Purified from rabbit serum. |
| |
| Species reactivity: | Human
|
| |
| Applications: | ICC, IP, WB
|
| |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1:500)
Immunoprecipitation (1:100)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Detects a band of ~65kDa by Western blot. |
| |
| Purity Detail: | Protein A-affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Short Term Storage: | +4°C |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Western blot analysis of Cardif (human), pAb (AT107) (Prod. No. ALX-210-929): Lane 1: MW marker; Lane 2: HepG2; Lane 3: PALA; and Lane 4: HeLa. Additional bands observed probably represent isoforms or cleaved products of Cardif.
Please mouse over
Product Literature References
MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep.
32, 108628 (2021),
Application(s): Western Blot,
Abstract;
Full Text
DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection: S. Pattabhi, et al.; J. Interferon Cytokine Res.
39, 331 (2019),
Abstract;
The 14-3-3η chaperone protein promotes antiviral innate immunity via facilitating MDA5 oligomerization and intracellular redistribution: J.P. Lin, et al.; PLoS Pathog.
15, e1007582 (2019),
Abstract;
Full Text
Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling: J. Hertzog, et al.; Eur. J. Immunol.
48, 1120 (2018),
Abstract;
Full Text
Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy: G.L. McLelland, et al.; Elife
7, e32866 (2018),
Abstract;
Full Text
NSs Protein of Sandfly Fever Sicilian Phlebovirus Counteracts Interferon (IFN) Induction by Masking the DNA-Binding Domain of IFN Regulatory Factor 3: J.D. Wuerth, et al.; J. Virol.
92, e01202-18 (2018),
Abstract;
Full Text
Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion: J. Gouttenoire, et al.; PLoS Pathog.
14, e1007471 (2018),
Abstract;
DDX3 directly regulates TRAF3 ubiquitination and acts as a scaffold to coordinate assembly of signalling complexes downstream of MAVS: L. Gu, et al.; Biochem. J.
474, 571 (2017),
Abstract;
Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68: Y. Rui, et al.; J. Virol.
91, e00546-17 (2017),
Abstract;
Full Text
NLRX1 promotes immediate IRF1-directed antiviral responses by limiting dsRNA-activated translational inhibition mediated by PKR: H. Feng, et al.; Nat. Immunol.
18, 1299 (2017),
Abstract;
Full Text
Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system: P. Bellecave, et al.; Hepatology
51, 1127 (2010),
Abstract;
Cleavage of the IPS-1/Cardif/MAVS/VISA does not inhibit T cell-mediated elimination of hepatitis C virus non-structural 3/4A-expressing hepatocytes: G. Ahlen, et al.; Gut
58, 560 (2009),
Abstract;
Antiviral suppression vs restoration of RIG-I signaling by hepatitis C protease and polymerase inhibitors: Y. Liang, et al.; Gastroenterology
135, 1710 (2008),
Abstract;
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol.
82, 335 (2008),
Abstract;
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ.
15, 1804 (2008),
Abstract;
Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J.
25, 3257 (2006),
Abstract;
General Literature References
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol.
27, 1 (2006),
Abstract;
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature
437, 1167 (2005),
Abstract;
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell
122, 669 (2005),
Abstract;
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol.
6, 981 (2005),
Abstract;
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell
19, 727 (2005),
Abstract;
Related Products
