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Caspase-1 polyclonal antibody



ALX-210-804-C100	100 µg	435.00 USD
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Product Details

Alternative Name:	IL-1β converting enzyme, Interleukin-1β converting enzyme, ICE

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to aa 224-242 (CEPH²²⁴KTSDSTFLVFMSHGIREG²⁴²) of human caspase-1.

UniProt ID:	P29466

Species reactivity:	Human, Mouse, Rat

Applications:	ICC, IHC, IP, WB

Recommended Dilutions/Conditions:	Immunocytochemistry (10-20µg/ml) Immunohistochemistry (1:50) Immunoprecipitation (4-8µg/ml) Western Blot (0.5-4µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects bands of ~45kDa (procaspase-1) and ~20kDa (cleavage product) by Western blot.

Purity Detail:	Epitope-affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5mM EDTA, and 0.03% Proclin.

Handling:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

Caspase-1 polyclonal antibody Western blot

Figure: Western blot analysis of caspase-1 expression in rat liver tissue lysate using Caspase-1, pAb (Prod. No. ALX-210-804)."

Caspase-1 polyclonal antibody Immunohistochemistry

Immunohistochemistry staining of human lung tumor tissue using Caspase-1, pAb (Prod. no. ALX-210-804) at a 1:50 dilution showed positive staining in both cytoplasm and nucleus of lung epithelial cells. Images were taken at magnification of 20 x (A) and 63 x (B).

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Product Literature References

Protein-Bound Uremic Toxins Induce Reactive Oxygen Species-Dependent and Inflammasome-Mediated IL-1β Production in Kidney Proximal Tubule Cells: M. Mihajlovic, et al.; Biomedicines 9, 1326 (2021), Abstract;

FANCA and FANCC modulate TLR and p38 MAPK-dependent expression of IL-1β in macrophages: M.R. Garbati, et al.; Blood 122, 3197 (2013), Abstract;