Product Details
Alternative Name: | C-C chemokine receptor 8, CD198 |
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Host: | Goat |
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Immunogen: | Synthetic peptide corresponding to aa 12-36 (V12TDYYYPDIFSSPCDAELIQTNGKL36) of the N-terminal extracellular domain of human CCR8 (C-C chemokine receptor 8). |
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UniProt ID: | P51685 |
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Species reactivity: | Human
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Applications: | ELISA, Flow Cytometry, IHC, WB
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Recommended Dilutions/Conditions: | ELISA (1:50,000)
Flow Cytometry (2-6µl per 106 cells/50-100µl)
Immunohistochemistry (1:125)
Western blot (1:500)
Optimal conditions must be determined individually for each application. |
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Purity Detail: | Epitope-affinity purified IgG. |
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Formulation: | Affinity purified antibody in 10mM KHPO4, 140mM NaCl, 1mg/ml BSA and 0.1% sodium azide. |
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Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Protocol: | Immunohistochemistry on paraffin sections: - Fix paraffin sections in 10% formalin. - De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 min. at room temperature (RT). - Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block non-specific binding sites. - Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 min. at RT. - Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 min. - Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 min. - Wash slides and incubate with DAB for 5-7 min. at RT. - Wash slides with PBS and counter stain with hematoxylin. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
CCL16 activates an angiogenic program in vascular endothelial cells: M. Strasly, et al.; Blood
103, 40 (2004),
Abstract;
The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque; Blood
97, 39 (2001),
Abstract;
CC Chemokine I-309 is the Principal Monocyte Chemottractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque; Circulation
102, 786 (2000),
Abstract;
The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem.
273, 386 (1998),
Abstract;
Full Text
Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol.
26, 3021 (1996),
Abstract;