Product Details
| Alternative Name: | Apo-2L, TNFSF 10, CD253 |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human soluble TRAIL (aa 95-281). |
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| UniProt ID: | P50591 |
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| GenBank ID: | U57059 |
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| Gene/Protein Identifier: | 8743 (Entrez GeneID) |
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| Source: | Purified from serum. |
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| Species reactivity: | Human
|
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| Specificity: | Recognizes soluble TRAIL. |
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| Crossreactivity: | Does not cross-react with mouse TRAIL. |
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| Applications: | Flow Cytometry, ICC, IHC (FS), WB
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| Purity Detail: | Protein A-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Schematic structure of the immunogen.
Figure 2: Detection of recombinant human TRAIL (Prod. No.
ALX-522-003) but not of recombinant human FasL (Prod. No.
ALX-522-001).
Method: Recombinant soluble TRAIL, and recombinant soluble FasL as negative control, were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and detected with PAb to TRAIL (human) (Prod. No.
ALX-210-732) at a dilution of 1:500. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system."
Figure 3: Immunostaining of HEK 293T cells transfected with a expression plasmid coding for human TRAIL (left panels), or mock transfected (right panel).
Method: 3 days after transfection of cells as indicated, cells were fixed with 4% formaldehyde or acetone for 5 min. at RT. Slides were blocked with IgG, and incubated for 1 hour with PAb to TRAIL (Prod. No.
ALX-210-732) at a dilution of 1:100 or control rabbit IgG in PBS containing 1% BSA. After washes with PBS, samples were incubated with a biotinylated secondary antibody to rabbit IgG for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC."
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Product Literature References
Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion: H. Dong, et al.; Nat. Med.
8, 793 (2002),
Abstract;
Hypericin photo-induced apoptosis involves the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and activation of caspase-8: C.M. Schempp, et al.; FEBS Lett.
493, 26 (2001),
Abstract;
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