Product Details
| Alternative Name: | C-C chemokine receptor 4, CD194 |
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| Host: | Goat |
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| Immunogen: | Synthetic peptide corresponding to aa 8-26 (D8TTQDETVYNS
YYFYESMP26C) of the N-terminal extracellular domain of mouse CCR4 (C-C chemokine receptor 4). |
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| UniProt ID: | P51680 |
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| Species reactivity: | Mouse
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| Crossreactivity: | Does not cross-react with human CCR4. |
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| Applications: | ELISA, Flow Cytometry, ICC, IHC (PS), WB
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| Recommended Dilutions/Conditions: | ELISA (1:100,000)
Flow Cytometry (1-3µl per 2x105 cells per 50µl)
Immunocytochemistry (1:500)
Immunohistochemistry (1:500)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Epitope-affinity purified F(ab`)2 fragment. |
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| Quality Control: | Immunocytochemistry on mouse spleen cells. |
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| Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide. |
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| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Mouse CCR4 is the homolog of human CCR4 receptor (85% identical), binding macrophage inflammatory protein-1α (MIP-1α) and RANTES. Mouse CCR4 transcripts are expressed in the thymus and in T-cell lines. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Immunohistochemistry on paraffin section of mouse spleen with CCR4 (mouse), pAb (Prod. No. ALX-210-621).
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Product Literature References
CD4+CD25+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype: Z. Guo, et al.; Int. Immunol.
20, 307 (2008),
Application(s): Flow cytometry,
Abstract;
CC-chemokine ligand 17 gene therapy induces tumor regression through augmentation of tumor-infiltrating immune cells in a murine model of preexisting CT26 colon carcinoma: N. Kanagawa, et al.; Int. J. Cancer
121, 2013 (2007),
Application(s): Flow cytometry,
Abstract;
IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge: S.A. Khader, at al.; Nat. Immunol.
8, 369 (2007),
Application(s): Flow cytometry,
Abstract;
Alloantigen-presenting plasmacytoid dendritic cells mediate tolerance to vascularized grafts: J.C. Ochando, et al.; Nat. Immunol.
7, 652 (2006),
Application(s): Flow cytometry,
Abstract;
Induction of surface CCR4 and its functionality in mouse Th2 cells is regulated differently during Th2 development: Y. Morimoto, et al.; J. Leukoc. Biol.
78, 753 (2005),
Application(s): Flow cytometry,
Abstract;
Role of CCR4 ligands, CCL17 and CCL22, during Schistosoma mansoni egg-induced pulmonary granuloma formation in mice: C. Jakubzick, et al.; Am. J. Pathol.
165, 1211 (2004),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4: A. Hoogewerf, et al.; BBRC
218, 337 (1996),
Abstract;
General Literature References
Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4: A. Hoogewerf, et al.; BBRC
218, 337 (1996),
Abstract;
